Abstract 【Objective】 To explore the effects of X ray irradiation on inflammatory factors expression and biological function in mouse microglia cell line-BV2. 【Methods】 BV2 cells were irradiated by 8 Gy X ray. After irradiation,the expression of inflammatory factors,M1 and M2 cell surface markers were detected with RT-qPCR and flow cytometry. The phagocytic ability of irradiated BV2 cells were evaluated using fluorescein-labeled particles co-culture. The time course changes of MAPKs' (mitogen-activated protein kinase) phosphorylation in irradiated BV2 cells were investigated with Western blotting. Comparison of the proteomic difference between irradiated and unirradiated BV2 cells were performed by mass spectrum identification and bioinformatics analysis. 【Results】 The gene expression of inflammatory factors like IL-1β,TNF-α and the M1 cell surface marker CD86 were significantly upregulated in irradiated cells. Meanwhile,X ray irradiation enhanced BV2 cells' phagocytic ability and increased the phosphorylation level of MAPKs pathway. 【Conclusion】 X ray irradiation drives BV2 cells to M1-like activation and promotes inflammation through the phosphorylation of MAPK pathway.
ZHANG Hai-ping,LIANG Dong,LI Wei. Effect of X Ray Irradiation on Inflammatory Factors Expression and Biological Functions in BV2 Cells[J]. JOURNAL OF CLINICAL RESEARCH, 2023, 40(3): 332-336.
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