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| Effects of miR-21-5p on Proliferation, Migration, Invasion and STAT3 Signaling Pathway of Multiple Myeloma Cells |
| BAI Tao, LU Liang, GUO Xiao-ning, et al |
| Department of Orthopedics, Yangling Demonstration Zone Hospital, Yangling Shaanxi 712100 |
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Abstract 【Objective】 To investigate the effects of microRNA (miR)-21-5p on the proliferation, migration, invasion, signal transduction and activator of transcription 3 (STAT3) signal pathway of multiple myeloma (MM). 【Methods】 Human MM RPMI-8226 cells were cultured in vitro and divided into control group (without transfection), siRNA NC group (transfected with nonsense siRNA) and miR-21-5p siRNA group (transfected with miR-21-5p siRNA). The expressions of miR-21-5p and STAT3 in RPMI-8226 cells were detected by real-time fluorescence quantitative PCR (qRT-PCR); The proliferation of RPMI-8226 cells was detected by CCK-8 method; The migration and invasion of RPMI-8226 cells were detected by Transwell method; Western blot (WB) was used to detect the expression of p-STAT3, STAT3, Ki-67 and matrix metalloproteinase-9 (MMP-9) in RPMI-8226 cells.【Results】 Compared with the control group and the siRNA NC group, the expression levels of miR-21-5p, STAT3 mRNA, cell OD value, number of migrating cells, number of invasive cells, p-STAT3/STAT3, Ki-67 and MMP-9 protein in RPMI-8226 cells in the miR-21-5p siRNA group were significantly lower (P<0.05). 【Conclusion】 Down-regulating the expression of miR-21-5p can inhibit the proliferation, migration and invasion of MM RPMI-8226 cells, and block the activation of STAT3 signaling pathway.
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Received: 15 December 2021
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2022, Vol. 39 
