医学临床研究
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JOURNAL OF CLINICAL RESEARCH  2021, Vol. 38 Issue (5): 687-690    DOI: 10.3969/j.issn.1671-7171.2021.05.014
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Expression and Significance of miR-125b in Ovarian Cancer Cells
GU Cai-ru, LIU Wei, LIN Meng-yuan
Department of Gynecology, Guangzhou Red Cross Hospital, Jinan University, Guangzhou, Guangdong 510220, China
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Abstract  【Objective】 To investigate the effect and mechanism of miR-125b on energy metabolism and proliferation in ovarian cancer cells. 【Methods】 Real-time PCR was used to detect the expression level of miR-125b in human ovarian epithelial cells and ovarian cancer cells. The proliferation of ovarian cancer cells was recorded by CCK-8. Glucose uptake and lactic acid production in ovarian cancer cells were measured by 3H labeled 2-deoxyglucose (3H-2DG) method and lactic acid quantitative kit. Western blot was performed for the expression of hexokinase 2 (HK2) in ovarian cancer cells after miR-125b-mimics overexpression. 【Results】 The real time PCR results showed that the expression level of miR-125b in ovarian cancer cells was significantly lower than that in normal ovarian epithelial cells (P<0.05). The CCK-8 results demonstrated that the proliferation capacity of ovarian cancer cells was significantly reduced after transfected with miR-125b-mimics for 48h and 72h compared with the control group (P<0.05). The quantitative results of 3H-2DG and lactic acid showed that glucose intake and lactic acid production were significantly reduced after overexpression of miR-125b in ovarian cancer cells (P<0.05); and aerobic glycolysis was significantly inhibited. Western blot results showed that the expression level of HK2 protein decreased after overexpression of miR-125b in ovarian cancer cells (P<0.05). 【Conclusion】 The low expression of miR-125b in ovarian cancer cells inhibits the energy metabolism and proliferation of ovarian cancer cells by down-regulating the expression of HK2.
Key wordsOvarian Neoplasms/PA      MicroRNAs /AN      Hexokinase/AN     
Received: 15 June 2020     
PACS:  R737.31  
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GU Cai-ru
LIU Wei
LIN Meng-yuan
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GU Cai-ru,LIU Wei,LIN Meng-yuan. Expression and Significance of miR-125b in Ovarian Cancer Cells[J]. JOURNAL OF CLINICAL RESEARCH, 2021, 38(5): 687-690.
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http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2021.05.014     OR     http://journal07.magtech.org.cn/yxlcyj/EN/Y2021/V38/I5/687
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