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| Role of TLRs-NF-κΒ Signaling Pathway in Children with Severe Pneumonia |
| YU Jie, WU Zhi-yuan, LI You-yi, et al |
| Department of paediatrics,Maternal and Child Health Hospital of Zengcheng district, Guangdong, 511300,China |
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Abstract 【Objective】 To investigate the role of Toll-like receptor-nuclear transcription factor-κΒ (TLRS-NF-κΒ) signaling pathway in children with severe pneumonia. 【Methods】 A total of 120 children with severe pneumonia from April 2019 to December 2019 were selected as observation group; A total of 89 healthy children were selected as the control group. The levels of interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α) and C-reactive protein (CRP) in children were determined by enzym-linked immunosorbent assay (ELISA). The percentage of TLRS cells with restricted presence type (TLR2, TLR4) positive cells was determined by flow cytometry. The Pearson correlation analysis software was used to analyze the correlation between the positive rates of TLR2 and TLR4 and the levels of HMGB1, TNF-α and CRP. 【Results】 The levels of serum IL-6, IL-8, TNF-α and CRP in children with severe pneumonia in the observation group were higher than those in control group (P<0.05). The results of flow cytometry showed that the percentage of TLR2 positive cells in the observation group was (59.30±5.71) %, and the percentage of TLR4 positive cells in the observation group was (59.70±9.48) %, which were both higher than that in the control group (0.20±0.06) %, and the percentage of TLR4 positive cells was (0.40±09) % (P<0.05). Pearson correlation analysis showed that TLR4 and TLR2 in TLRS-NF-κΒ signaling pathway were positively correlated with the levels of inflammatory factors IL-6, IL-8, TNF-α and CRP in children with severe pneumonia (P<0.05). 【Conclusion】 TLRS-NF-κΒ signaling pathway is highly expressed in children with severe pneumonia, which is strongly correlated with inflammatory factors. Strengthening TLRS-NF-κΒ signaling pathway determination can guide clinical diagnosis and treatment.
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Received: 21 January 2021
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2021, Vol. 38 
