槲皮素对脂多糖诱导的小鼠急性肺损伤的影响及其机制

doi:10.3969/j.issn.1671-7171.2021.05.003

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Abstract 【Objective】 To investigate the effect of quercetin (QUE) on acute lung injury (ALI) induced by lipopolysaccharide (LPS) in mice and study its mechanism. 【Methods】 Sixty male C57bl/6 mice were randomly divided into 4 groups (15 in each group): normal group (CON group), LPS group and QUE treatment group. The ALI mice were constructed by intraperitoneal injection of LPS (10 mg/kg). The CON group was given the same amount of normal saline, while the QUE treatment group were given QUE 25 mg/kg 2 hours before the administration of LPS. The mice in both CON group and The LPS group were given the same amount of DMSO. HE staining was used to detect lung histopathological changes. Western blotting was used to detect the expression of PERK and key proteins in the apoptosis pathway. Lung tissue oxidative stress levels were detected with malondialdehyde(MDA). Counted Inflammatory cells in bronchoalveolar lavage fluid (BLAF) and myeloperoxidase (MPO) activity in lung tissue were used to detect with inflammatory response. 【Results】 Quercetin alone had no effect on the morphology and inflammation of lung tissue in mice. Intraperitoneal injection of LPS induced pathological damage of mouse lung tissue which increased the number of total cells and neutrophils in BALF, increased MPO activity, and increased the levels of TNF-α and IL-1β in lung tissue. However, the QUE prevention group and the QUE treatment group significantly alleviated the above changes. Western blotting results showed that QUE pretreatment inhibited the expression of proteins related to the endoplasmic reticulum stress PERK (double-stranded RNA-dependent protein kinase ER kinase) signaling pathway and reduced the expression of caspase-3, a key protein in the apoptosis pathway. 【Conclusion】 Quercetin can reduce ALI in mice induced by LPS by inhibiting endoplasmic reticulum stress (ERS) and reducing oxidative stress and inflammation. The mechanism is related to the inhibition of PERK signaling pathway of ERS.

Key words： Acute Lung Injury Mice, Nude Quercetin/TU Lipopolysaccharides/PD

Received: 22 March 2021

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R256.1

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http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2021.05.003 OR http://journal07.magtech.org.cn/yxlcyj/EN/Y2021/V38/I5/647

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