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| Expression of Slit2 and Robo1 Proteins and their Relationship with Microvessel Density in Pancreatic Carcinoma |
| LIANG Gang, WANG Zhi-xiang, HE Meng-guo,et al |
| Department of Hepatobiliary Surgery, Nuclear Industry 215 Hospital of Shaanxi,Xianyang 712000,China |
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Abstract 【Objective】To investigate the expression of Slit2 and Robo1 proteins in pancreatic cancer and their relationship with microvessel density (MVD).【Methods】 Immunohistochemical staining SP method was used to detect the expression of Slit2 and Robo1 proteins in 30 cases of pancreatic cancer tissues and 30 cases of adjacent tissues. The expression changes of Slit2 protein and Robo1 protein under different clinicopathological conditions were observed. The microvessel density (MVD) marker CD105 was detected to study the relationship between Slit2 /Robo1 protein expression and MVD in pancreatic cancer. 【Results】The positive rates of Slit2 and Robo1 were 60.00% and 70.00% in pancreatic cancer tissues, respectively; and the positive rates in the adjacent tissues were 33.33% and 40.00%, respectively. The differences between the two types of tissues were statistically significant (P<0.05). There was a positive correlation between the positive expression of Slit2 protein and Robo1 protein (r=0.818, P< 0.05). The expression of MVD in pancreatic cancer tissues was higher than that in the adjacent tissues (t=9.475, P<0.05). The MVD in pancreatic cancer tissues with positive expression of Slit2 and Robo1 was much higher than that in pancreatic cancer tissues with negative expression of Slit2 and Robot1 (t=2.048, P<0.05,t=2.897, P<0.05). Expression of Slit2, Robo1 and MVD were significantly associated with tumor size, pathological type, clinical stage, and lymph node metastasis of pancreatic cancer (P<0.05).【Conclusion】 The increased expression of Slit2 protein and Robo1 protein in pancreatic cancer tissue is closely related to the growth, invasion and metastasis of pancreatic cancer. The mechanism may be that Slit2/Robo1 signaling pathway promotes the occurrence and development of pancreatic cancer by inducing tumor angiogenesis. The expression intensity of Slit2 /Robo1 and the enumerations of MVD can be used to predict the progression and prognosis of pancreatic cancer.
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Received: 28 October 2019
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2020, Vol. 37 
