|
|
|
| Lentivirus-mediated shRNA Vector Targeted Knockout of ZNF217 Gene Expression on Invasion and Apoptosis of Human Neuroblastoma KELLY Cells |
| TANG Xiang-lian, WEN Jia-bing, LI Ming, et al |
| Hunan children's Hospital, Changsha 410007, Hunan |
|
|
|
|
Abstract 【Objective】To investigate the effect of targeted knockout of ZNF217 gene expression by shRNA vector on invasion and apoptosis of human neuroblastoma Kelly . 【Methods】The shRNA hairpin structure of ZNF217 gene was constructed by siRNA technology. The human neuroblastoma cell line Kelly cells were divided into blank control group (group A), blank vector virus group (group B) and ZNF217 gene silencing expression vector lentivirus group (Group C). The expression levels of ZNF217, caspase-3 and caspase-9 were detected by RT-PCR and Western blot. Transwell kit was used to detect cell invasion, AV-PI kit to detect apoptosis, CCK-8 kit to detect cell proliferation. 【Results】The transfection titer of lentivirus was 3×107 TU/mL, and the transfection efficiency was over 92%. RT-PCR and Western blot showed that the relative expression of ZNF217 mRNA and protein in the group C was lower than that in the group A and B (P<0.05). However, the relative expression of Caspase-3 and caspase-9 in the group C was higher than that in the group A and B (P<0.05). The cell proliferation rate of the group C was significantly lower than that of the group B and the group A (P<0.05), and the cell invasion ability of the group C was significantly lower than that of the group B and the group A (P<0.05). The apoptosis rate of the group C was significantly higher than that of the group B and the group A (P<0.05). 【Conclusion】Targeted knockout of ZNF217 gene expression by shRNA vector can inhibit the proliferation, invasion and apoptosis of human neuroblastoma cell line Kelly cells, so ZNF217 gene can be used as a potential therapeutic target for neuroblastoma.
|
|
Received: 26 September 2019
|
|
|
|
|
|
[1] 李勇, 肖雅玲, 黎明, 等. Apelin/APJ在小儿神经母细胞瘤中的表达及意义[J].临床小儿外科杂志, 2019,18(2): 154-158.
[2] Zhang J, Zhuo ZJ, Wang J,et al. CASC15 gene polymorphisms reduce neuroblastoma risk in Chinese children[J].Oncotarget,2017, 8(53):91343-91349.
[3] Bellanger A, Donini C F, Vendrell J A, et al. The critical role of the ZNF217 oncogene in promoting breast cancer metastasis to the bone[J].J Pathol,2017, 242(1):73-89.
[4] Zhai J, Liu J, Cheng X, et al. Zinc finger gene 217 (ZNF217) Promoted Ovarian Hyperstimulation Syndrome (OHSS) through Regulating E2 Synthesis and Inhibiting Thrombospondin-1 (TSP-1) [J].Sci Rep,2017, 7(1):3245-3261.
[5] 常世川, 李涛, 李刚,等. 锌指蛋白217在非小细胞肺癌组织中的表达及其临床意义[J].中国现代医学杂志, 2018,28(10):55-59.
[6] Zherdeva V, Kazachkina NI, Shcheslavskiy V, et al. Long-term fluorescence lifetime imaging of a genetically encoded sensor for caspase-3 activity in mouse tumor xenografts[J].J Biomed Opt,2018, 23(3):1-11.
[7] Rukoyatkina N, Butt E, Subramanian H, et al. Protein kinase A activation by the anti-cancer drugs ABT-737 and thymoquinone is caspase-3-dependent and correlates with platelet inhibition and apoptosis[J].Cell Death Dis,2017, 8(6):e2898.
[8] Pu X, Storr SJ, Zhang Y, et al. Caspase-3 and caspase-8 expression in breast cancer: caspase-3 is associated with survival[J].Apoptosis,2017, 22(3):357-368.
[9] Chen H, Tang X, Zhou B, et al. A ROS-mediated mitochondrial pathway and Nrf2 pathway activation are involved in BDE-47 induced apoptosis in Neuro-2a cells[J].Chemosphere,2017, 184(2):679-687.
[10] Yan X, Wang L, Yang X, et al. Fluoride induces apoptosis in H9c2 Cardiomyocytes via the mitochondrial pathway[J].Chemosphere,2017, 182(11):159-166.
[11] Hooper S, Gaggioli C, Sahai E. A chemical biology screen reveals a role for Rab21-mediated control of actomyosin contractility in fibroblast-driven cancer invasion[J].Br J Cancer,2010, 102(2):392-402. |
| [1] |
. [J]. JOURNAL OF CLINICAL RESEARCH, 2019, 36(9): 1763-1765. |
|
|
|
|
2019, Vol. 36 
