|
|
|
| Effect of Butylphthalide on Renal Interstitial Fibrosis in Rats with Single-side Ureteral Obstruction |
| AN Na, Sun Jian-ping |
| Medical College of Qingdao University,Qingdao 266021,China |
|
|
|
|
Abstract Objective To observe the effects of butylphthalide(NBP) on the renal interstitial fibrosis in rats with unilateral ureteral obstruction(UUO) and to explore the possible mechanism. Methods A total of 54 clean level male rats were selected to establish the UUO models. These rats were randomly divided into three groups with 18 in each group: group A and B with unilateral ureteral obstruction (left ureteral ligation in close proximity to renal pelvis) and group C without UUO (procedure mimic as control group). Rats in group A received NBP 180 mg/kg/d lavage, while rats in group B received equal dosage saline lavage. Rats in group C were only isolated the ureter with no ligation but give equal saline lavage as well. Rat kidney and blood samples were taken at postoperative d2, d3, and d7, and renal lipid peroxide reaction markers superoxide dismutase (T-SOD) and malondialdehyde (MDA) activities were measured. Results Compared to d2, the levels of T-SOD at postoperative d3 and d7 in group A, group B and group C decreased, while the level of MDA increased (P<0.05). T-SOD level was decreased and MDA level was increased in all three groups at d7 after surgery, if compared to d3 (P<0.05). Compared to group A, the level of T-SOD in group B was decreased, while the level of MDA was increased at postoperative d3 and d7 (P<0.05). Compared with group C, the level of T-SOD was decreased and the level of MDA was increased in group A at postoperative d3 and d7 (P<0.05). The levels of type I collagen protein in group A and group B at postoperative d3 and d7 were significantly higher than those in their matched group at postoperative d2 (P<0.05). Further, the levels of type I collagen protein in group B were greater than those in group A. The postoperative d3 and d7 of group B at postoperative d3 and d7 were higher than those of group A (P<0.05). Type I collagen was expressed in a small amount in group C, and there was no significant difference in the levels of d2, d3, and d7 in group C (P>0.05).Conclusion NBP plays a significant role in the regulation of renal interstitial fibrosis in rats in unilateral ureteral obstruction, which may be related to the regulation of the activity of MDA and T-SOD.
|
|
Received: 03 April 2018
|
|
|
|
|
|
[1] 李华, 王保兴. 肥大细胞在肾间质纤维化中作用机制的新进展[J]. 中华临床医师杂志:电子版, 2013, 7(10):142-143.
[2] Ni H, Chen J, Pan M, et al. FTY720 prevents progression of renal fibrosis by inhibiting renal microvasculature endothelial dysfunction in a rat model of chronic kidney disease.[J]. J Mol Hist, 2013, 44(6):693-703.
[3] 刘其锋, 叶建明. EMT与肾间质纤维化[J]. 中国中西医结合肾病杂志, 2013, 14(5):561-562.
[4] Moon JA , Kim HT , Cho IS, et al .IN-1130, a novel transfor- ming growth factor-beta type I receptor kinase (ALK5)inhibi-tor, suppresses renal fibrosis in obstructive nephropathy[ J] .Kidney Int , 2006, 70(7):1234-1243.
[5] 耿金平, 长春艳, 张超,等. 肾间质纤维化的中医诊疗进展[J]. 医学信息, 2015, 28(7):339-340.
[6] Wang J, Liang M, Xu J, et al. Renal expression of advanced oxidative protein products predicts progression of renal fibrosis in patients with IgA nephropathy[J]. Lab Invest, 2014, 94(9):966-977.
[7] 樊威伟. 肾间质纤维化主要信号通路与治疗的研究进展[J]. 湖南中医杂志, 2016, 32(7):191-193.
[8] 彭国璇, 蔡颜嵘, 李名浩,等. 肾间质纤维化相关信号通路的研究进展[J]. 现代生物医学进展, 2015, 15(24):4794-4796.
|
|
|
|
2019, Vol. 36 
