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| Effects of Matrix Metalloproteinase-10 and Insulin-like Growth Factor-1 on Metastasis of Histiocytosarcoma |
| PENG Fang, ZHONG Bi-ling, CHEN Yan-yu, et al |
| Department of Pathology, the Second Provincial Hospital of Guangdong, Guangzhou, 510317, China |
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Abstract 【Objective】To establish a mouse histiocytosarcoma model and screen a high invasive tumor strain. The expression of IGF-1 and MMP-10 protein in the tumor strain and clinical specimens were detected and the clinical significance was explored. 【Methods】 Primary L-II tumor cells were inoculated subcutaneously into the axilla of the mice, and the lung metastases were taken to make a suspension to inoculate another mouse, and then the lung metastases were taken for further passage. A mouse xenograft model was established using primary cells and ninth generation cells, respectively. The survival time and tumor size of primary tumor and metastatic tumor were compared. Immunohistochemistry and Western blot were used to detect the expression of IGF-1 and MMP-10. 【Results】 Mouse histiocytic sarcoma L-II to passage 9 were screened by serial transfer in vivo. The results showed that the selected tumor strains grew faster than the pre-screened tumor strains. The survival time of mice before and after screening was 29.23±2.90 days and 23.66±2.97 days, respectively. The difference was significant (P<0.05). The expression of IGF-1 and MMP-10 protein in the 9th generation tumor strain of mice was up-regulated compared with the expression of the tumor strain before screening. 【Conclusion】 The expressions of IGF-1 and MMP-10 were both enhanced in the mouse model of the 9th generation tumor cells, which suggests that IGF-1 and MMP-10 may play a role in the process of histiocytosarcoma metastasis.
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Received: 25 September 2018
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[1] 段瑞.WHO造血与淋巴组织肿瘤分类(2016)[J].诊断病理学杂志, 2017,24(12):956-958.
[2] Riggi N, Aguet M, Stamenkovic I. Cancer metastasis: A reappraisal of its underlying mechanisms and their relevance to treatment[J].Annu Rev Pathol,2018,13:117-140.
[3] 彭芳.应用基因芯片技术筛选小鼠组织细胞肉瘤L-Ⅱ转移相关基因[J].热带医学杂志, 2013,13(2):171-174.
[4] Klupp F, Neumann L, Kahlert C, et al. Serum MMP7, MMP10 and MMP12 level as negative prognostic markers in colon cancer patients[J].BMC Cancer,2016,16:494.
[5] 王海燕, 权毅, 王艳, 等. 新辅助化疗对乳腺癌患者血清胰岛素样生长因子-1的影响[J].医学临床研究, 2016,33(5):928-929,932.
[6] Li L, Li JC, Yang H, et al. Expansion of cancer stem cell pool initiates lung cancer recurrence before angiogenesis[J].Proc Natl Acad Sci USA,2018,115(38):E8948-E8957.
[7] 李爱科, 李青山, 林萍萍, 等. HIF-1α、VEGF及MMP-9在非小细胞肺癌组织中的表达及其意义[J].医学临床研究, 2017,34(9):1687-1690. |
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2019, Vol. 36 
