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| Expression and Significance of miR-29b and miR-126 in Iris Tissues of Diabetic Neovascular Glaucoma |
| JI Xue-ying, SUN Hong-liang, LI Chuan-wu, et al |
| Department of Ophthalmology, First Affiliated Hospital of Hainan Medical College, Haikou Hainan 571012 |
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Abstract 【Objective】 To observe the expression of micro RNA-29b (miR-29b) and micro RNA-126 (miR-126) in the iris tissues of diabetes mellitus (DM) neovascular glaucoma (NVG) in order to explore their significance in the pathogenesis and development of DM with NVG. 【Methods】One hundred cases of DM complicated with NVG were selected as DM-NVG group, 54 cases of typically simple glaucoma were selected as glaucoma group (NVG group) and 15 cases of healthy iris tissue donors were as control group (CON group). The expression levels of miR-29b and miR-126 were detected by fluorescence quantitative polymerase chain reaction (PCR). The relationship between the two genes' expression with the clinicopathological characteristics of patients with DM combined with NVG was analyzed. Pearson correlation analysis was used to analyze the relationship between miR-29b and miR-126 expression in the iris tissue of patients. Logistic multiple regression analysis was used to analyze the risk factors of NVG in DM patients. 【Results】The expression of miR-29b in the iris tissues of the DM-NVG group and NVG group was significantly lower than that of the control group (P<0.05). However, the expression of miR-126 in the DM-NVG and NVG groups was significantly higher than that of the CON group (P<0.05). The expression of miR-29b in the iris of DM-NVG group was significantly lower than that of the NVG group (P<0.05), and its expression of miR-126 was significantly higher than that of the NVG group (P<0.05). The expression of miR-29b in iris tissues was related to intraocular pressure, visual acuity index and VEGF (P<0.05). The expression of miR-126 was related to intraocular pressure and VEGF (P<0.05). There was a significant negative correlation between miR-29b and miR-126 expression in iris tissues of patients with DM combined with NVG (P<0.001). Multivariate regression analysis showed that VEGF, DM type, miR-29b and miR-126 were the risk factors for DM complicated with NVG (P<0.05). 【Conclusion】The expression of miR-29b is low in the iris tissues of diabetic neovascular glaucoma, while miR-126 is highly expressed, which may be involved in regulating the occurrence and development of diabetic neovascular glaucoma. Thus,it provides a reference for clinical treatment of diabetic neovascular glaucoma.
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Received: 03 December 2018
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2019, Vol. 36 
