Abstract【Objective】To investigate the effect and related molecular mechanism of AKIP1 on the apoptosis of human colorectal cancer cells HCT-116. 【Methods】AKIP1 siRNA were designed and transfected into HCT-116 cells. Real-time PCR and Western Blot were used to detect the expression of AKIP1 mRNA and protein, respectively. Cell viability and apoptosis were measured by MTT and flow cytometry. We detected the mRNA expression of apoptosis associated genes by Real-time PCR, and the protein expression of NF-κB p65 in the cytoplasm and nucleus and the apoptosis-related proteins were detected by Western Blot. 【Results】After transfection with AKIP1 siRNA, the expression levels of AKIP1 mRNA and protein were significantly down-regulated. MTT assay showed that AKIP1 siRNA significantly inhibited the viability of HCT-116 cells. Flow cytometry showed that AKIP1 siRNA significantly promoted apoptosis of HCT-116 cells. Meanwhile, AKIP1 siRNA up-regulated Bax, cleaved Caspase-3, and down-regulated bcl-2 expression. In addition, AKIP1 siRNA down-regulated the expression of NF-κB p65 in the nucleus and up-regulated the expression of NF-κB p65 in the cytoplasm. 【Conclusion】AKIP1 may inhibit the apoptosis of HCT-116 cells by activating NF-κB p65 and promote the progression of colorectal cancer.
SUN-Jing,SU Cheng-xun. Effect of AKIP1 on Apoptosis of Colorectal Cancer Cells and Its Molecular Mechanism[J]. JOURNAL OF CLINICAL RESEARCH, 2019, 36(5): 839-842.
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2019, Vol. 36 
