U0126对高糖作用下角膜上皮细胞活性的影响

doi:10.3969/j.issn.1671-7171.2019.01.016

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Abstract 【Objective】 To investigate the effect of U0126 on activity of human corneal epithelial cells (HCEC) induced by high glucose. 【Methods】 HCEC was divided into normal group (5.5 mmol/L glucose), high glucose group (50 mmol/L glucose) and U0126 low, medium and high concentration group (5, 10, 20 micron o/L, U0126 + 50 mmol/L glucose), and cultured for 48 hours. Cell viability, apoptosis, ROS fluorescence intensity, calcium concentration, mitochondrial membrane potential, aspartate proteolytic enzyme 3 (Caspase 3) and Caspase 9 activity, and the expression of Bcl-2, Bax and Cyt C protein were measured.【Results】 Compared with the high glucose group, the cell viability of U0126 low, medium and high concentration groups was significantly increased (P<0.01), the apoptotic rate, ROS fluorescence intensity, calcium concentration and Caspase 3, 9 activity were decreased (P<0.01), the mitochondrial membrane potential was increased (P<0.01), the expression of Bcl-2 was up-regulated (P<0.01), the expression of Cyt C in cytoplasm was down-regulated (P<0.01), and the expression of Bax in U0126 medium and high concentration groups was down-regulated (P<0.01).【Conclusion】 U0126 may resist apoptosis of HCEC cells induced by high glucose through mitochondrial pathway.

Key words： Epithelium Corneal/PA

Received: 27 April 2018

PACS:

R361.3

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	Articles by authors
	ZHU Xi-yu
	WANG Xiao-ping
	MAO Xiao-chun
	et al

Cite this article:

ZHU Xi-yu,WANG Xiao-ping,MAO Xiao-chun, et al. Effect of U0126 on Corneal Epithelial Cell Activity Induced by High Glucose[J]. JOURNAL OF CLINICAL RESEARCH, 2019, 36(1): 52-54.

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http://journal07.magtech.org.cn/yxlcyj/EN/10.3969/j.issn.1671-7171.2019.01.016 OR http://journal07.magtech.org.cn/yxlcyj/EN/Y2019/V36/I1/52

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