颞叶癫痫小鼠慢性期的条件恐惧记忆变化及海马神经元树突棘的形态改变

doi:10.3969/j.issn.1671-7171.2018.09.012

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Abstract 【Objective】 To investigate the changes of fear conditioning memory and the morphological changes of dendritic spines in hippocampal neurons in the chronic phase of mice with temporal lobe epilepsy.【Methods】 Temporal lobe epilepsy models were induced by intraperitoneal injection of Kainic acid (KA) in mice. The mice were divided into two groups: KA group (n=8) which received KA and the control group (CN,n=4) which were intraperitoneal injection of saline instead. After two months, emotional learning and memory were evaluated by fear-conditioning test, and changes in dendritic spines of hippocampal neuron in both groups of mice were observed by Golgi Staining. 【Results】 In the contextual test, the percentage of connective fear memory freezing time in the KA group was (17.50±0.88)%, which was significantly lower than that in the CN group (60.24±3.01)%, and the difference was statistically significant (P<0.05). In the tone cued test, the percentage of freezing time in the sound-connected fear memory of the KA group was (49.01±2.45)%, which was significantly lower than that of the CN group (79.57±3.98)%, and the difference was statistically significant (P<0.05). Compared to the control group, Golgi staining showed that the density of dendritic spines in hippocampal neurons in KA group was significantly reduced. The results of image analysis showed that the dendritic spine length of hippocampal neurons and the density of dendritic spines of hippocampal neurons in KA group were (2.1±0.11) μm, (10±0.5)/10 μm, respectively, which were significantly lower than that of CN group. (2.5±0.13) μm, (12±0.6)/10 μm, respectively, and the differences were statistically significant (P<0.05).【Conclusion】 The mice with KA-induced temporal lope epilepsy in chronic phase have the impaired fear conditioning memory, which may be associated with the morphological changes of of dendritic spines in hippocampal neurons.

Key words： Mice Inbred C57BL/AB Conditioning (Psychology) Nerve Degeneration

Received: 14 August 2017

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http://www.jcr.net.cn/EN/10.3969/j.issn.1671-7171.2018.09.012 OR http://www.jcr.net.cn/EN/Y2018/V35/I9/1703

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