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| Effect of lncRNA BC002811 on Invasion and Metastasis of Gastric Cancer Cells |
| YANG Xiang-ron, WEI Chun-shan, LIU Chan, et al |
| The Second People's Hospital of Longgang District, Shenzhen, Guangdong, 518112 China |
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Abstract 【Objective】 To detect the expression of lncRNA BC002811 in gastric cancer tissues and cell lines, and to study its function regulating the migration and invasion in gastric cancer cells. 【Methods】 Real-time PCR was used to detect the expression of BC002811 in gastric cancer tissues and cell lines. Gastric cancer cell stable strains with overexpression and down-regulation of lncRNA BC002811were established. Subsequently, the migration and invasion capability of different strains were analyzed with Scratch (wound-healing) test and transwell assay, respectively. Finally, real-time quantitative PCR and Western blot were used to investigate the molecular mechanism of BC002811 to promote tumor progression.【Results】 The relative expression of lncRNA BC002811 was significantly up-regulated in gastric cancer tissues and cell lines compared to corresponding adjacent normal tissues and normal gastric cell line, respectively(P<0.05). When lncRNA BC002811 was over-expressed, the migration and invasion ability of gastric cancer cells were enhanced and the expression of matrix metalloproteinase-2 (MMP2), MMP9 and Vimentin (one of the mesenchymal cell markers) increased. While the expression of E-cadherin (one of the epithelial cell markers) decreased(P<0.05). When the expression of lncRNA BC002811 was inhibited, the opposite results occurred. 【Conclusion】 lncRNA BC002811 promoted the migration and invasion in gastric cancer cells and contributed to the tumor deterioration.
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Received: 28 February 2018
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2018, Vol. 35 
