miR-423-5p在卵巢癌组织中的表达及对癌细胞增殖的影响

doi:10.3969/j.issn.1671-7171.2018.09.004

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Abstract 【Objective】 To evaluate the expression of miR-423-5p in ovarian cancer tissues and investigate the effect of miR-423-5p on the proliferation of ovarian cancer cells. 【Methods】 The expression of miR-423-5p in 25 pairs of ovarian cancer and adjacent tissue was determined by real-time PCR. The expression of miR-423-5p in four ovarian cancer cell lines (SK-OV-3, A2780, COC1 and OVCAR3) was measured as well. The miR-423-5p-low-expressed cells were transfected with miR-423-5p mimic or NC mimic and the miR-423-5p-high-expressed cells were transfected with miR-423-5p inhibitor or NC inhibitor. Cell proliferation activities were examined by MTT assay. The expression level of ING-4 (growth inhibitory gene 4) was measured by real-time PCR. Dual-Luciferase reporter assay was also used to examine whether or not ING-4 was a direct target of miR-423-5p. 【Results】 The level of miR-423-5p was significantly increased in ovarian cancer tissues compared to cancer-adjacent tissue (P<0.05). A2780 cell showed the lowest level and OVCAR3 cell showed the highest level of miR-423-5p among the four ovarian cancer cell lines. A2780 cells transfected with miR-423-5p mimic significantly increased cell proliferation activity while also remarkably inhibited the expression of ING-4 compared to NC mimic transfected A2780 cells. In contrast, OVCAR3 cells after transfection with miR-423-5p inhibitor significantly inhibited cell proliferation and increased ING-4 level when compared to NC inhibitor transfected cells. Dual-Luciferase reporter assay showed that when compared to wt-ING-4 3'UTR+NC mimic group, the relative luciferase activity was significantly increased in wt-ING-4 3'UTR+miR-423-5p mimic group (P<0.05). However, there were unchanged in mut-ING-4 3'UTR+NC mimic group and mut-ING-4 3'UTR+miR-423-5p mimic group. 【Conclusion】 miR-423-5p is closely related to the occurrence and development of ovarian cancer. It may may be a novel target in the prevention and treatment of ovarian cancer.

Key words： Ovarian Neoplasms/PP MicroRNAs Cell Proliferation

Received: 08 June 2018

PACS:

R737.31

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	SONG Yang
	REN Fang

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SONG Yang,REN Fang. Effect of miR-423-5p Expression on the Proliferation of Ovarian Cancer Cells[J]. JOURNAL OF CLINICAL RESEARCH, 2018, 35(9): 1675-1678.

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http://www.jcr.net.cn/EN/10.3969/j.issn.1671-7171.2018.09.004 OR http://www.jcr.net.cn/EN/Y2018/V35/I9/1675

[1] Miller KD, Siegel RL, Lin CC,et al. Cancer treatment and survivorship statistics, 2016[J].CA Cancer J Clin,2016, 66(4):271-289.
[2] Sethi S, Sethi S, Bluth MH. Clinical implication of microRNAs in molecular pathology: an update for 2018[J].Clin Lab Med,2018, 38(2):237-251.
[3] Bagnoli M, Canevari S, Califano D, et al. Development and validation of a microRNA-based signature (MiROvaR) to predict early relapse or progression of epithelial ovarian cancer: a cohort study[J].Lancet Oncol,2016, 17(8):1137-1146.
[4] Motohashi K.A simple and efficient seamless DNA cloning method using SLiCE from Escherichia coli laboratory strains and its application to SLiP site-directed mutagenesis[J].BMC Biotechnol,2015, 15:47.
[5] Yang H, Fu H, Wang B, et al. Exosomal miR-423-5p targets SUFU to promote cancer growth and metastasis and serves as a novel marker for gastric cancer[J].Mol Carcinog,2018 ,57(9):1223-1236.
[6] 6.Lu X, Lu J.The significance of detection of serum miR-423-5p and miR-484 for diagnosis of colorectal cancer[J].Clin Lab,2015, 61(1-2):187-190.
[7] Zhu Y, Li T, Chen G, et al.Identification of a serum microRNA expression signature for detection of lung cancer, involving miR-23b, miR-221, miR-148b and miR-423-3p[J].Lung Cancer,2017, 114:6-11.
[8] Sun X, Wang M, Liu H et al. MicroRNA-423 enhances the invasiveness of hepatocellular carcinoma via regulation of BRMS1[J].Am J Transl Res,2017, 9(12):5576-5584.
[9] Li S, Zeng A, Hu Q, et al. miR-423-5p contributes to a malignant phenotype and temozolomide chemoresistance in glioblastomas[J].Neuro Oncol,2017, 19(1):55-65.
[10] Wan Y, Fei X, Wang Z, et al. miR-423-5p knockdown enhances the sensitivity of glioma stem cells to apigenin through the mitochondrial pathway[J].Tumour Biol,2017, 39(4):1010428317695526.
[11] Gambari R, Brognara E, Spandidos DA,et al. Targeting oncomiRNAs and mimicking tumor suppressor miRNAs: New trends in the development of miRNA therapeutic strategies in oncology (Review) [J].Int J Oncol,2016, 49(1):5-32.
[12] Coles AH, Jones SN.The ING gene family in the regulation of cell growth and tumorigenesis[J].J Cell Physiol,2009, 218(1):45-57.
[13] Yuan S, Jin J, Shi J, et al. Inhibitor of growth-4 is a potential target for cancer therapy[J].Tumour Biol,2016, 37(4):4275-4279.

[1]	. [J]. JOURNAL OF CLINICAL RESEARCH, 2018, 35(9): 1861-1863.