CD32抗体诱导产生的人CRP对小鼠急性肾损伤自噬的研究

doi:10.3969/j.issn.1671-7171.2018.08.019

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Abstract 【Objective】 To invest the effect of human C- reactive protein on autophagy in acute kidney injury . 【Methods】 In order to induce the production of human CRP in mice, 25 μ g/mL body volume was injected intraperitoneally 2 hours before operation (body blood volume was equal to the gram weight of mice multiplied by 0.08 ) of anti CD32 antibody, then the acute renal ischemia-reperfusion injury model was established in mice. The mice were divided into Sham group, wild type mice group (CRP-Wt-I/R group) and transgenic mice expressing human CRP gene group(CRP-Tg-I/R group); the changes of CRP in mice before and after renal ischemia-reperfusion injury 2 hours before and 0h、24h、72h after operation were compared, Kidney specimens were obtained at 24 h and 72 h after operation. After HE staining, the pathological changes of renal tissue were observed, the expression of autophagy associated protein Beclin-1 was detected by immunohistochemical method, and the autophagy of renal tissue was observed under transmission electron microscope.【Results】 At 24 hours and 72 hours after renal ischemia-reperfusion injury, the expression of Beclin-1 protein in renal tubular epithelial cells of the CRP-Tg-I/R group decreased significantly (P<0.01), and no autophagy was observed.【Conclusion】 Human CRP down-regulated autophagy activity in renal tissue of AKI.

Key words： Kidney Diseases/TH Disease Models Animal Receptors IgG C-Reactive Protein

Received: 02 July 2018

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R965.1

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	JIJin-ling
	TANGQian-qiu
	ZHANGXiao-yun

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JIJin-ling,TANGQian-qiu,ZHANGXiao-yun. Effect of Human C- reactive Protein Induced by Anti-CD32 Antibody on Autophagy of Acute Kidney Injury in Mice[J]. JOURNAL OF CLINICAL RESEARCH, 2018, 35(8): 1513-1516.

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http://www.jcr.net.cn/EN/10.3969/j.issn.1671-7171.2018.08.019 OR http://www.jcr.net.cn/EN/Y2018/V35/I8/1513

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