苯甲酰胺对实验大鼠牙周炎炎症细胞和炎症因子水平的影响

doi:10.3969/j.issn.1671-7171.2018.06.009

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Abstract 【Objective】To study the effects of poly ADP-ribose polymerase (PARP) inhibitor Benzamide on the levels of inflammatory cells and inflammatory cytokines in experimental periodontitis of rats. 【Methods】 Fourty male Wistar rats ( SPF grade,3-month-old) of periodontitis model were randomly divided into periodontitis group, periodontitis intervention group, drug control group and blank group, with 10 mice in each group. The rat model of periodontitis was established by ligature of the first maxillary molar with stainless steel wire. At the fifth week after modeling, the periodontitis intervention group and the drug control group were intraperitoneally injected with 0.3 mg / kg PARP inhibitor Benzamide, while the periodontitis group and the blank group were intraperitoneally injected with equal doses of normal saline. Three rats in each group were randomly sacrificed at the end of 4,5 and 6 weeks. The histopathological changes of periodontal tissue were observed with H＆E staining.The levels of serum hs-CRP、IL-1β、IL-6 and TNF-α were measured by ELISA.Results were analyzed by using SPSS 17.0, and P<0.05 was considered statistically significant. 【Results】Periodontitis intervention group showed that, at the 5th and 6th weeks, there were slightly migration of cells from the gingival epithelium toward the root, a shallow periodontal pocket, and a small amount of inflammatory cell infiltration in the gingival epithelium, the groove epithelium and the lamina propria. In the same time period, the periodontitis group showed periodontal vascular dilatation and hyperemia, migration of gingival epithelium cells to the root, a deep periodontal pocket, and a large number of inflammatory cell infiltration in the gingival epithelium, under the groove epithelium and underlying lamina propria. The drug control group and the blank group showed that the epithelial cells were located at the border of glazed cementum and that the periodontal membrane fibers were arranged in an orderly manner without inflammatory cell infiltration. Serum levels of hs-CRP, IL-1β, IL-6 and TNF-α in the drug control group and the blank group at week 5 and 6 were significantly lower than those in periodontitis intervention group and periodontitis group (P<0.05). The periodontitis intervention group was significantly lower than the periodontitis group, but there was no significant difference between the drug control group and the blank group (P>0.05).【Conclusion】 Activation of PARP aggravates the symptoms of periodontitis in the pathogenesis of periodontitis. Benzoamide relieves periodontal lesions by inhibiting PARP, which reduces the inflammatory cells and inflammatory cytokines in the periodontium in experimental rats.

Key words： Periodontitis/DT Rats Benzamides/AD InflammationMediators

Received: 29 March 2018

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R-332

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	LI Xiao-hong
	XU Ying-hong
	XING Kong-cai

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LI Xiao-hong,XU Ying-hong,XING Kong-cai. Effect of Benzamide on Inflammatory Cells and Inflammatory Factors in Periodontitis in Experimental Rats[J]. JOURNAL OF CLINICAL RESEARCH, 2018, 35(6): 1066-1068.

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http://www.jcr.net.cn/EN/10.3969/j.issn.1671-7171.2018.06.009 OR http://www.jcr.net.cn/EN/Y2018/V35/I6/1066

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