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| Expression of MiR-34b and Target Gene CDK6 in Osteosarcoma |
| HUANG Cheng-xiao, YU Hua-long, GAO Chao, et al |
| Department of Orthopaedics Ⅲ, Dongfeng Hospital, Hubei University of Medicine, Shiyan Hubei 442008 |
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Abstract 【Objective】The aim of this study was to investigate the expression and clinical significance of miR-34b and target gene cyclin-dependent kinase 6 (CDK6) in osteosarcoma, and to provide a new therapeutic approach for gene therapy of osteosarcoma. 【Methods】A total of 51 cases of osteosarcoma patients who underwent surgical resection in our hospital from March 12, 2016 to March 12, 2017 were selected as the subject of study. There were 51 cases of osteosarcoma and corresponding 51 cases of normal bone tissue (At least 2 cm from the edge of the cancer tissue) were collected. The levels of miR-34b and CDK6 mRNA in osteosarcoma and adjacent normal tissues were detected by qRT-PCR. The expression of CDK6 protein in osteosarcoma and adjacent normal tissues was detected by immunohistochecmistry (S-P method). The relationship between the expression of miR-34b and CDK6 was analyzed, and the correlation between the expression of the two genes and their clinicopathological features of osteosarcom was analyzed as well. 【Results】 The expression level of miR-34b mRNA in osteosarcoma tissue was 0.34±0.16, which was significantly lower than that in adjacent tissues (1.06±0.13); the difference was statistically significant (Z=24.942,P=0.000). The expression level of CDK6 mRNA in osteosarcoma tissue was 4.37±0.46, which was significantly higher than that in adjacent tissue (1.03±0.12, Z=50.174, P=0.000). The positive expression rate of CDK6 protein in osteosarcoma tissue was 78.43%, which was significantly higher than that in adjacent tissue 17.65%(χ2=37.744,P=0.000). The expression of miR-34b and CDK6 was negatively correlated (r=-0.483, P=0.000). The expression of miR-34b and CDK6 were not significantly correlated with age, sex, tumor size and tumor location of osteosarcoma patients (Z=0.203、0.384、0.230、0.135;P=0.840、0.703、0.819、0.894.χ2=0.644、0.051、2.403、0.746;P=0.422、0.821、0.121、0.689), while itwas closely related to Enneking staging and tumor metastasis (Z=2.509、3.962, P=0.016、0.000;χ2=6.451、4.217,P=0.040、0.040). 【Conclusion】miR-34b may be involved in the development of osteosarcoma through regulating the expression of target gene CDK6 gene.
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Received: 29 January 2018
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2018, Vol. 35 
