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| Effect of Dexmedetomidine on Hepatic Ischemia Reperfusion Injury in Cholestatic Rats |
| LONG Ying-xi, ZOU Yi, CHEN Wen-yan, et al |
| Hunan Provincial People's Hospital, Changsha, Hunan 410005, China |
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Abstract 【Objective】To investigate the effect of dexmedetomidine (Dex) on hepatic ischemia reperfusion injury (IRI) in cholestatic rats.【Method】A total of 24 cholestatic rats modeled by bile duct ligation were randomly divided into 3 groups (n=8): sham operated group (A group), hepatic IRI group (B group) and Dex group (C group). The hepatic portal triad were clamped by Pringle maneuver and maintained for 30 minutes. After 4 hours of reperfusion, blood samples were obtained for testing levels of total bilirubin (TBIL), direct billirubin (DBIL), alanine transaminase (ALT), aspartate transaminase (AST), and tumor necrosis factor-α (TNF-α). Liver tissues homogenates were obtained to test values of superoxide dismutase (SOD) and malondialdehyde (MDA), and were observed after hematoxylin-eosin (HE) staining for histopathological assessment.【Results】After 4 hours of reperfusion, TBIL and DBIL levels in three groups were not significantly different (P>0.05). Serum ALT, AST and TNF-α levels, tissue MDA contents in the B group were significantly higher than those in the A group (P<0.05). Tissue SOD activities in the B group were significantly lower than the A group (P<0.05). Serum AST and TNF-α levels, tissue MDA contents in the C group were significantly lower than the B group (P<0.05). Histopathological changes (liver tissue destruction, hepatic cord disorders and inflammatory cell infiltration) in the B group were more obvious than in the A and C groups.【Conclusion】Dexmedetomidine may attenuate hepatic IRI injury in cholestatic rats, possibly by alleviating systemic inflammation and oxidative stress.
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Received: 13 April 2017
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. [J]. JOURNAL OF CLINICAL RESEARCH, 2018, 35(4): 761-762. |
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2018, Vol. 35 
