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| Effect of Schwann Cells on Repairing Rabbit Radius Defect with Tissue Engineered Bone Combined with Osteoblasts |
| LIAN Feng, CUI Yong, CHEN Hui-chao, et al |
| The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China |
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Abstract 【Objective】To observe the effect of Schwann cells on repairing radius defects with bone tissue engineering scaffolds composited with osteoblasts in rabbit.【Methods】Bone marrow mesenchymal stem cells (BMSCs) were isolated and cultured, then induced to differentiate into osteoblasts (IOBs). Schwann cells (SCs) were extracted from neonatal rabbit, which were identified by S-100 immunofluorescence. New Zealand rabbits were used to establish the model of radial defect. The models were randomly divided into 5 groups. group A was blank control group, while bone defects were implanted with nano-chitosan/hydroxyapatite porous scaffolds in group B. and bone defects were implanted with osteoblasts and scaffolds in group C. Furthermore, bone defects were implanted with Schwann cells and scaffolds in group D. and bone defects were implanted with Schwann cells, osteoblasts and scaffolds in group E. After 8 weeks, immunohistochemistry, HE staining, gross morphology and X-ray were performed to evaluate the formation of new bone in the radial defect of rabbits in each group.【Results】The results of X-ray examination after 8 weeks postoperatively showed that the bone defect was partially healed in B, C and D groups. Groups C and D showed better effects than group B. The bone defect in group E was healed completely, while the bone defect in group A weren't healed at all. Compared to group B or C or D after 8 weeks, HE staining showed that the number of osteoblasts and trabecular meshwork were the highest in group E. Also, bone repair was the fastest in group E. Immunohistochemistry showed that the expression of BDNF and NGF in group E were significantly higher than other groups.【Conclusion】Schwann cells (SCs) can significantly promote the repair of rabbit radius bone defects by engineering scaffold composited with osteoblasts, which was closely related to the expression of BDNF and NGF.
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Received: 15 November 2017
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2018, Vol. 35 
