外耳道乳头状瘤与HPV感染的病理学及原位杂交研究

doi:10.3969/j.issn.1671-7171.2018.04.024

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Abstract 【Objective】To investigate the etiology and pathogenesis of papilloma of external auditory canal (PEAC), and analyze the relationship between the PEAC and human papilloma virus (HPV) infection.【Methods】From January 2011 to December 2016, 130 PEAC surgical specimens were collected from our hospital. The infection of HPV6 / 11 DNA and HPV16/18 DNA infection were detected by HE staining and in situ hybridization.【Results】The low-risk HPV6/11 DNA infection rate of PEAC with koilocyte (79.52%) was significantly higher than that of PEAC without koilocyte (P<0.05), while the HPV16/18 DNA infection rate of PEAC with half koilocyte (17.54%) was higher than that of PEAC without koilocyte, but the difference was not statistically significant (0) (P>0.05). The HPV6/11DNA infection rate in men was significantly higher than that in women (80.21% vs 47.06%), but there was no statistically significant difference between men and women in the HPV16/18 DNA infection rate (21.87% vs 14.71%). HE staining showed that squamous epithelium was papillary hyperplasia, mild or severe hyperkeratosis of epidermis, parakeratosis cells scattered in epithallium, lymphocytes infiltration in central connective tissues, a large number of koilocytes in epidermal superficial or middle-level cells and dermal papilla hyperplasia and dilatation of capillaries. The results of in situ hybridization found that HPV6/11 positive cells showed positive nucleus, while HPV16/18 positive cells showed positive cytoplasm.【Conclusion】The etiology and pathogenesis of PEAC may due to HPV virus infection.

Key words： Ear Neoplasms/PA Ear Neoplasms/GE Papilloma/PA Papilloma/GE Papillomaviridae Tumor Virus Infections/PA In Situ Hybridization

Received: 05 April 2017

PACS:

R764.14

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	CHENG Mo-yu
	WANG Xu

Cite this article:

CHENG Mo-yu,WANG Xu. Pathological and In Situ Hybridization Study of HPV Infection in Papilloma of External Auditory Canal[J]. JOURNAL OF CLINICAL RESEARCH, 2018, 35(4): 696-699.

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http://www.jcr.net.cn/EN/10.3969/j.issn.1671-7171.2018.04.024 OR http://www.jcr.net.cn/EN/Y2018/V35/I4/696

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