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| Research on the Effect and Mechanism of Cofilin-1 on Invasion, Migration and Adhesion in Human Glioma Cells |
| ZHAO Yun-dong, SUN Dong-tao, CHEN Zhen-jun |
| The Second Affiliated Hospital of Shenyang Medical College, Liaoning, Shenyang 110035 |
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Abstract 【Objective】To investigate the effects and possible molecular mechanisms of interfering Cofilin-1 gene expression on the invasion, migration and cell adhesion in human glioma cells.【Methods】The RNAi technique was used to interfere with the expression of Cofilin-1 gene in human glioma cell line SHG-44. The interference effect of Cofilin-1 was verified by Real-time PCR and Western blot. Scratch assay was used to detect cell migration ability. Transwell chamber assay was used to detect cell invasion ability. Adhesion experiment was used to detect cell adhesion ability. The expression of ICAM-1 and NF-κB p65 protein in cytoplasm and nucleus were detected by Western blot.【Results】RNAi interference successfully inhibited the expression of Cofilin-1 in SHG-44 cells. Interference of Cofilin-1 expression inhibited the migration, invasion and adhesion in SHG-44 cells, decreased the expression of ICAM-1 and the expression of NF-κB p65 in nucleus.【Conclusion】Interference of Cofilin-1 expression can inhibit the migration, invasion and adhesion in glioma cells, and may play a role by regulating the activation of NF-κB pathway. Cofilin-1 may be a potential therapeutic target for gliomas.
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Received: 26 February 2018
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2018, Vol. 35 
