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| Study of the Correlation between the Distribution of Pathogens in Nasopharyngeal and Middle Ear Cavities in Children with Chronic Secretory Otitis Media |
| SONG Gui-lin, LI Xiang-sheng, PENG Ya, et al |
| Department of ENT, The Fourth People’s Hospital of Changsha, Changsha 410006, China |
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Abstract 【Objective】To analyze the distribution of pathogens and its correlation with middle ear fluid (MEF) and nasopharyngeal secretion (NPS) in children with chronic secretory otitis media (SOM) .【Methods】MEF and NPS secretions were collected from SOM children. The pathogen distribution of NPS and MEF were observed by PCR technique for detection of A.otitidis, S.pneumonia, H.influenzae, M.catarrhails, S.aureus, and S.viridans. The correlation was evaluated.【Results】1) The presence of S.pneumonia or S.aureus in middle earfiuid was significantly positively correlation with the presence of the same species in the nasopharynx (S.pneumoniae, P=0.000, V=0.638; S.aureus, P= 0.000, V=0.761) . Higher prevalence (Sensitivity, specificity and positive predictive values) was found for S.pneumonia and S.aureu S.H.influenzae or M.catarrhails in middle earfiuid correlated positively with the presence of the same species in the nasopharynx, however, the correlation was low (H.influenzae, P=0.021, V=0.258 M.catarrhails, P=0.000, V=0.459) . 2) Alloiococcus otitidis and Streptococcus viridans were not significantly associated with MEF and NPS PCR result S.3) The highest prevalence in NPS was found for H.influenzae (79.63%) , followed by S.aureus (75.93%) and S.pneumonia (75.93%) . The highest prevalence in MEF was found for S.pneumonia (75.93%) and S.aureus (75.93%) .【Conclusion】The results indicate that PCR results from NPS are valuable for prediction of the pathogen presence in MEF in children with SOM.
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Received: 22 August 2017
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2017, Vol. 34 
