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| Adverse effects of PM2.5 on HaCaT Cells and Relevant Mechanisms |
| XIONG Shu-han, WU Yue-ting, MENG Fan-zhu, et al |
| Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun 130021, China |
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Abstract 【Objective】To explore the effects of PM2.5 on HaCaT cells and whether or not PDTC will alleviate the adverse effects caused by PM2.5.【Methods】HaCaT cells were co-cultured with PM2.5, PDTC, PM2.5+PDTC for 24 hours, respectively. HaCaT cells without any treatment were set as control. The content of malondialdehyde (MDA), reactive oxygen species (ROS) level, the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px)_ and the expression of NF-kB in the cells from different groups were measured and compared.【Results】Cell viability decreased with the increasing of PM2.5 concentrations, the cell viabilities of 200, 400 and 800 μg/ml groups were significantly lower than the normal group (P<0.05). Compared with PM2.5 group, the MDA contents, the level of ROS and the expression of NF-kB of the cells treated with PM2.5+PDTC, or PDTC, or vehicle treated cells were significantly lower while the activities of SOD and GSH-Px were significantly higher (P<0.05). Furthermore, the levels of MDA, ROS and NF-κB p65 protein in PM2.5 + PDTC group were significantly higher than those in control group and PDTC group【Conclusion】PM2.5 was able to decrease the cell viability and cause oxidative damage to HaCaT cells while PDTC can alleviate the damages to certain extent.
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Received: 14 March 2017
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[1]Hui H,Qi Y,Xi L,et al.Air Pollution and Control in Different Areas of China[J].Crit Rev Env Sci Tec,2010,40(6):452-518.
[2]Kathuria P,Silverberg JI.Association between small particle air pollution,climate and childhood eczema prevalence and severity:a US population-based study[J].Pediatr Allergy Immunol,2016,27(5):478-485.
[3]Bowatte G,Lodge C,Lowe AJ,et al.The influence of childhood traffic-related air pollution exposure on asthma,allergy and sensitization:a systematic review and a meta-analysis of birth cohort studies[J].Allergy,2015,70(3):245-256.
[4]Magnani ND,Muresan XM,Belmonte G,et al.Skin damage mechanisms related to airborne particulate matter exposure[J].Toxicol Sci,2016,149(1):227-236.
[5]Han X,Liang WL,Zhang Y,et al.Effect of atmospheric fine particles on epidermal growth factor receptor mRNA expression in mouse skin tissue.[J].Genet Mol Res,2016,15(1):1-5.
[6]Jun-Ji C,Zhen-Xing S,Chow JC,et al.Winter and summer PM2.5 chemical compositions in fourteen Chinese cities.[J].J Air Waste Manage,2012,62(10):1214-1226.
[7]Errol M Thomson,Dalibor Breznan,Subramanian Karthikeyan,et al.Contrasting biological potency of particulate matter collected at sites impacted by distinct industrial sources[J].Part Fibre Toxicol,2016,13:65.
[8]Rafael B,Ron S,Hussein A,et al.A protective effect of pyrrolidine dithiocarbamate in a rat model of liver cirrhosis[J].Liver Int,2004,24(2):169-176. |
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2017, Vol. 34 
