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| Expression and Role of IL-13 in Mice with Acute Lung Injury |
| CAO Jian-bin |
| Department of thoracic surgery, Liuzhou Hospital,Guang xi Liuzhou, 545006,China |
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Abstract 【Objective】 To investigate the expression of IL-13 (interleukin-13) and its effect on inflammatory reaction of acute lung injury (ALI) induced by LPS in mice.【Methods】LPS was intratracheally injected into mice to induce the ALI model. The real-time PCR and ELISA were used to detect IL-13 mRNA and protein expression in lung tissue, bronchoalveolar lavage fluid (BALF) and serum. The effects of IL-13 on ALI was investigated using IL-13 neutralizing antibody. The ELISA method was used to detect the content of TNF-α and IL-1β in BALF and serum. HE staining was used to observe pathological damage of lung tissue changes in mice.【Results】Compared with the control group, IL-13 mRNA expression in lung tissue and IL-13 protein expression in BALF and serum were increased markedly in ALI mice induced by LPS (P<0.05). Pretreatment of IL-13 neutralizing antibody increased the expression of TNF-α and IL-1β, and aggravated the changes in pathological damage of ALI mice (P<0.05).【Conclusion】 IL-13 expression increases in ALI induced by LPS. While inhibition of IL-13 aggravates the damage in mouse with ALI induced by LPS, which indicates that IL-13 plays a protective role in ALI endogenously.
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Received: 11 April 2017
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(本文编辑:詹道友) [收稿日期] 2017-04-11
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2017, Vol. 34 
