甘麦大枣汤对慢性应激抑郁模型大鼠学习记忆及海马突触可塑性的影响

doi:10.3969/j.issn.1671-7171.2017.06.007

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Abstract 【Objective】To investigate the effects of Ganmai Dazao Decoction on learning, memory and hippocampus synaptic plasticity in chronic depression model rats. 【Methods】Wistar rats were randomly divided into 4 groups: the control group, he depression model group, the fluoxetine group (5.4mg/kg) and the Ganmai Dazao Decoction (3.84 g/kg) group. Depression models were established by chronic mild unpredictable stress and solitude. The fluoxetine hydrochloride and Ganmai Dazao Decoction group were intra-gastrically administrated (ig) with corresponding drugs once a day for 21 days, and the depression model group was intraperitoneally injected with water. Then, we observed the effects of water maze test in model rats with Ganmai Dazao Decoction. The expression of SYN I (synaptophysin I), SYNA (synaptophysin), and BDNF (brain-derived neurotrophic factor) were detected by the immunohistochemistry and Western-Blotting methods. 【Results】Compared to the control group, the horizontal and vertical activity scores of the model group decreased significantly (P<0.01). However, the escape latency (EL) and target quadrant latency increased significantly (P<0.05), and the expression of SYN I, SYNA, BDNF were reduced dramatically as well. Compared to the outcomes of the model group, Ganmai Dazao Decoction reduced the EL (P<0.05), shortened the target quadrant latency (P<0.05) and increased the expression of hippocampal SYN I, SYNA and BDNF(P<0.01 or P<0.05) of the model rats.【Conclusion】Ganmai Dazao Decoction can significantly improve the learning and memory abilities in chronic depression model rats, which may play a role in the promotion of hippocampal synaptic plasticity.

Key words： GANMAI DAZAO DECOCTION/PD Disease Models, Animal Chronic Disease Depressive Disorder/DT Memory Neuronal Plasticity/DE Synapses/DE

Received: 10 February 2017

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R542.22

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	YANG Ming-yu
	MENG Pan
	YIN Tao
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YANG Ming-yu,MENG Pan,YIN Tao, et al. Effects of Ganmai Dazao Decoction on Learning, Memory and Hippocampus Synaptic Plasticity of Chronic Stress Depression Model Rats[J]. JOURNAL OF CLINICAL RESEARCH, 2017, 34(6): 1064-1068.

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http://www.jcr.net.cn/EN/10.3969/j.issn.1671-7171.2017.06.007 OR http://www.jcr.net.cn/EN/Y2017/V34/I6/1064

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(本文编辑:詹道友) [收稿日期] 2017-02-10