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| Anti-Proliferation and Pro-apoptosis Effects of Decitabine Combined with Idarubicin on AML Cells Harboring Mutant DNMT3A |
| LUO Zi-mian, TAN Zhen-qing, LOU Dian,et al |
| Department of Hematology, Xiangtan Central Hospital, Xiangtan 411100, Hunan Province, China |
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Abstract 【Objective】To evaluate the antiproliferation and pro-apoptotic effects of decitabine (DAC) combined with idarubicin (IDA) on OCI-AML3 cells harboring mutant DNMT3A.【Methods】OCI-AML3 cells were treated with different concentrations of IDA singly or combined with 2μM DAC for 48 h. Then, MTT and Annexin-V/PI were used to examine the proliferation and apoptosis of OCI-AML3 cells.【Results】IDA at relatively low concentrations (≤40nM) did not inhibit proliferation of OCI-AML3 cells significantly, but promoted proliferation inhibition remarkably when combined with 2μM DAC (P<0.01). DAC combined with IDA induced higher apoptotic rate compared to DAC or IDA as a single agent (P<0.001).【Conclusion】AML cells harboring mutant DNMT3A are primarily resistant to IDA at relatively low concentrations, while DAC can increase the susceptibility to IDA.
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Received: 07 February 2017
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(本文编辑:师晓阳) [收稿日期] 2017-02-07
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2017, Vol. 34 
