Abstract Objective To investigate the PPAR-γ,NF-κB,TNF-α, IL-10 expression,blood gas and the biochemical changes in double-hit rabbit animal model of multiple organ dysfunction syndrome (MODS). The effects of rosiglitazone and GW9662 were evaluated. Methods In this study, we prepared double-hit MODS rabbit model through fluid resuscitation after hemorrhagic shock and LPS stimulation. We used PPAR-γ agonist rosiglitazone and PPAR-γ antagonist GW9662 to regulate the expression of PPAR-γ. We detected the expression of PPAR-γ, NF-κB, TNF-α, and IL-10 in liver 24 hour after the double-hit MODS model was established. Biochemical and blood gas analysis at the same time were detected as well. Results The expressions of PPARγ mRNA in T-H group and D-H-S group were significant lower than that in sham group (P<0.05). In the D-H group, the expression of PPARγ mRNA with rosiglitazone application was higher than that in control group (P<0.05) whilethe expression of PPARγ mRNA with GW9662 application was significant lower than that in rosiglitazone-treated group(P<0.05). In the T-H group, the expressions of NF-κB and TNF-α increased significantly (P<0.05).However, the expressions of IL-10 in the T-H group did not change significantly as compared to sham group (P>0.05). In D-H-S group, the expressions of NF-κB and TNF-α increased significantly as compared to T-H group (P<0.05). The expressions of IL-10 in D-H-S group increased significantly as compared to sham group (P<0.05), but it did not change significantly compared to T-H group (P>0.05). In D-H -R group, the expressions of NF-κB and TNF-α decreased significantly compared with D-H-S group (P<0.05). The expressions of IL-10 increased significantly compared with D-H-S group (P<0.05). In D-H-G group, the expressions of NF-κB and TNF-α increased significantly compared with D-H-R group (P<0.05). The expressions of IL-10 decreased significantly compared with D-H-R group (P<0.05). In the T-H group, blood gas and biochemical parameters did not change significantly compared with sham group. In the D-H-S group, blood gas and biochemical parameters increased significantly compared with sham group and T-H group (P<0.05). In the D-H-R group, blood gas and biochemical parameters ameliorated significantly compared with D-H-S group (P<0.05). In the D-H-G group, blood gas and biochemical parameters deteriorated significantly compared with D-H-R group (P<0.05). Conclusion As the agonist of PPAR-γ, Rosiglitazone could improve the organ function in MODS rabbit induced by double-hit.
GU Chang-wei,QIAO Wan-hai,LIU Zhong. Rosiglitazone's Protective Effects on Multiple Organ Dysfunction Syndrome Induced by Double-Hit in Rabbits[J]. JOURNAL OF CLINICAL RESEARCH, 2017, 34(5): 882-885.
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