成骨诱导人脐带间充质干细胞复合nHA/PA66支架异位成骨作用的实验研究

doi:10.3969/j.issn.1671-7171.2017.04.013

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Abstract 【Objective】To explore the ability of ectopic bone formation of osteoblastic-induced hUCMSCs composite with nHA/PA66 material in nude mouse back and provide theories foundation for further large animal experiments of bone tissue engineering.【Methods】Human umbilical cord mesenchymal stem cells were seeded into nHA/PA66 scaffolds and cultured in vitro; HUCMSCs suspension, nHA/PA66 scaffold material, osteogenic-induced hUCMSCs and nHA/PA66 scaffold composite were implanted into the dorsal muscle pouch of nude mice; the ectopic bone formation was observed. The expression of HE staining and osteocalcin immunohistochemical staining were observed after operation, and the osteogenic ability was observed by scanning electron microscope.【Results】Neither osteoblastic-induced hUCMSCs nor nHA/PA66 material were sclerotized in the muscular pouch of nude mouse back. The osteoblastic-induced hUCMSCs + nHA/PA66 were maturely sclerotized in large amount,the material partly degradated.【Conclusion】 The biological properties of the composites prepared by the combination of hUCMSCs and nHA/PA66 scaffolds in the nude mice have ectopic osteogenesis.

Key words： Mesenchymal Stem Cells Umbilical Cord Osteoblasts Hydroxyapatites Tissue Engineering

Received: 03 May 2016

PACS:

R318.08

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	Articles by authors
	CHEN Gang
	LIAO Qian-de
	HU You-wei
	et al

Cite this article:

CHEN Gang,LIAO Qian-de,HU You-wei, et al. Study on Ectopic Bone Formation of Osteoblastic-Induced hUCMSCs Composite with nHA/PA66 Material[J]. JOURNAL OF CLINICAL RESEARCH, 2017, 34(4): 666-670,672.

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http://www.jcr.net.cn/EN/10.3969/j.issn.1671-7171.2017.04.013 OR http://www.jcr.net.cn/EN/Y2017/V34/I4/666

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(本文编辑:詹道友) [收稿日期] 2016-05-03