|
|
|
| Expression Level of CD8+ and CD4+ Memory T cells and Its Diagnostic Value in Patients with Active Tuberculosis and TB Latent Infection |
| YUAM Cheng-liang, LIU Zhao-hong, ZHANG Yao,et al |
| People’s Hospital of Deyang City 618000 |
|
|
|
Abstract 【Objective】To investigate the expression and significance of CD8+ and CD4+ memory T cells in active pulmonary tuberculosis and TB latent infection. 【Methods】Patients diagnosed with active tuberculosis and latent infection in our hospital were chosen as the subject. There were 25 cases of active pulmonary tuberculosis patients (TB group), 25 cases of latent tuberculosis infection (latent infection group), and 30 cases of healthy people (healthy group). CD4+ and CD8+ memory T cells in peripheral blood were detected by flow cytometry. The results were compared with patients' high-resolution CT scores (HRCT) to analyze the correlation between the two. 【Results】The level of CD4+ Tcm and CD8+ Tcm in the latent infection group was higher than that of the active pulmonary TB group and healthy group; the difference was statistically significant (P<0.05). Also, the expression of CD4+ and CD8+ Tcm in TB group was higher than that of the healthy group with statistical significance (P<0.05). On the other hand, the level of CD4+ Tem and CD8+ Tem in TB group and latent infection group was significantly lower than that in the healthy group; the difference was statistically significant (P<0.05). However, the differential expression in CD4+ Tem and CD8+ Tem between the patients with pulmonary tuberculosis and the patients with latent infection was not statistically significant (P>0.05). Furthermore, the level CD4+ Tcm and CD8+ Tcm in TB patients had significant negative correlation with HRCT score (P<0.05). However, in TB patients, the level of CD4+ Tem and CD8+ Tem level had no significant correlation coefficient (P>0.05) with HRCT score. 【Conclusion】Levels of Central memory T cells of CD4+ Tcm and CD8+ Tcm in the active and latent tuberculosis infection patients were significantly increased, which may reflect the degree of injury of pulmonary tuberculosis.
|
|
Received: 23 May 2016
|
|
|
|
|
|
[1] 原艳明,王仲元,王心静,等. 结核分枝杆菌抗原特异反应性白细胞介素-22在结核性胸膜炎鉴别诊断中的应用[J].现代仪器与医疗,2014,24(03):1-3.
[2] 鄢仁晴,方宁,赵建军,等. 肺结核患者外周血CD4+和CD8+记忆性T细胞亚群、IL-17、IL-27表达的初步探讨[J].中国免疫学杂志,2012,32(10):930-935.
[3] 鄢仁晴,赵兴容,赵玉洁,等. 结核性胸膜炎患者胸水记忆性T细胞亚型的研究[J].实用医学杂志,2012,26(14):2356-2358.
[4] 范任华,向延根,杨励,等. 多耐药肺结核患者特异性细胞免疫失衡与调节性T 细胞 CD4＋CD25＋CD127low有关[J].东南大学学报(医学版),2015,28(4):525-531.
[5] 郑建,欧勤芳,刘袁媛,等. 结核性胸膜炎患者结核特异性T淋巴细胞的免疫应答及其迁徙作用[J].中华传染病杂志,2013,31(12):715-718.
[6] Marin ND, Paris SC, Rojas M,et al.Reduced frequency of memory T cells and increased Th17 responses in patients with active tuberculosis[J].Clin Vaccine Immunol,2012,19(10):1667-1676.
[7] Papageorgiou CV, Anastasopoulos A, Ploussi M,et al.Flow cytometry analysis of CD4+IFN-γ+ T-cells for the diagnosis of mycobacterium tuberculosis infection[J].Cytometry Part B: Clinical Cytometry,2016,90(3):303-311.
[8] Chatterjee S, Clark CE, Lugli E,et al.Filarial infection modulates the immune response to Mycobacterium tuberculosis through expansion of CD4+ IL-4 memory T cells[J].J Immunol,2015,194(6):2706-2714.
[9] Torrado E, Fountain JJ, Liao M,et al.Interleukin 27R regulates CD4+ T cell phenotype and impacts protective immunity during Mycobacterium tuberculosis infection[J].J Exp Med,2015,212(9):1449-1463. |
|
|
|
2016, Vol. 33 
