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| Expression of miRNA-145 in Patients with Liver Cancer Induced by Hepatitis B Liver Cirrhosis and Its Relationship with Prognosis |
| YUAN Dong-hong, WANG Fang, TAN Yu-e ,et al |
| Department of Digestion Internal Medicine, the Affiliated Hospital of Yan'an University, Yan＇an 716000, Shaanxi, China |
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Abstract 【Objective】To study the expression of cancer tissue miRNA-145 in patients with liver cancer induced by Hepatitis B liver cirrhosis and its relationship with prognosis.【Methods】Seventy-four patients with liver cancer induced by hepatitis B cirrhosis who were admitted in our hospital between January 2009 and January 2012 were selected as the study objects. Cancer tissue specimens were collected from all patients during operation. The expression of miRNA-145 in tumor tissues, the real-time quantitative RT-PCR and cell proliferation were detected, and cell invasion assay was performed. According to miRNA-145 concentration , 50 nmol/L, 100 nmol/L and 150 nmol/L were sub-divided , and the control group was treated with liposome. The effect of miRNA-145 expression and its ability on proliferation of tumor cells, the impact of invasion and metastasis in patients with liver cancer induced by Hepatitis B liver cirrhosis and their relationships with prognosis were investigated and concluded.【Results】The expression levels of cancer tissues and miRNA-145 in Human hepatocellular carcinoma HepG2 cell line were higher than those in normal tissues, the difference was statistically significant (P<0.05). There was no significant difference in the relative proliferation (D490) in each group in 24 h (P>0.05); in 48h and 72h, the cell relative proliferation (D490) of miRNA-145 transfection in different concentration groups was lower than that in the liposome treated group, and the difference was statistically significant (P<0.05). The number of tumor cells through the cell membrane of the experimental group was (0.29±0.05),relatively lower than that of the liposome treated group(0.36±0.12), the difference was statistically significant (P<0.05). The survival time of patients with higher expression of miRNA-145 was longer than that in the lower expression group, and the difference was statistically significant (P<0.05).【Conclusion】The expression of miRNA145 can inhibit tumor proliferation, control tumor progression, reduce invasion and metastasis of tumor cells, and promote apoptosis of tumor cells, thus it is able to prolong the survival time of patients with liver cancer. It is worth clinical in-depth study.
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Received: 03 June 2016
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2016, Vol. 33 
