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| An Investigation on the Mechanism of Hydrogen SulfideInduced Delayed Preconditioning on Myocardial Ischemia Reperfusion Injury in Rats |
| Department of Anesthesiology, Second Yueyang Hospital, Yueyang 414000 |
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Abstract 【Objective】To investigate the mechanism of hydrogen sulfide delayed preconditioning on myocardial ischemia reperfusion injury in rats. 【Methods】Three groups (n=10 animals/group) of SpragueDawley male rats were studied: group S consisted of control rats that received no treatment, Group IR consisted of rats treated with NS (1.0 ml/kg iv) 24 h before ischemia, and Group H consisted of rats treated with NaHS (0.05 mg/kg) 24 h before ischemia. Group IR and H were subjected to ischemia by 30 min of coronary artery occlusion followed by 2 h of reperfusion. At the end of reperfusion, the plasma concentration of IL6 and IL10 were measured. The peroxisome proliferatoractivated receptor γ (PPARγ) was measured by Western blotting. 【Results】Compared with Group S, Group H and Group IR had higher levels of IL6 and IL10 (P<0.05). Compared with Group IR, Group H had lower levels of IL6 and higher levels of IL10 (P<0.05). Compared with Group S, Group H and Group IR had lower PPARγ protein expression in myocardium (P<0.05).Compared with Group IR, Group H had higher levels of PPARγ protein expression (P<0.05).【Conclusion】The hydrogen sulfide delayed preconditioning attenuates myocardial IR injury possibly through upregulating PPARγ expression in rats.
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Received: 26 March 2016
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2016, Vol. 33 
