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| Effects of Extracorporeal Photopheresis Treated Donor Lymphocyte Infusion on Graft Outcome After Murine Heart Transplantation |
| Department of kidney transplantation, Nanfang Hospital,Guangzhou, 510515 |
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Abstract 【Objective】 To investigate the effects of extracorporeal photopheresis（ECP） treated donor lymphocyte infusion on graft survival after murine heart transplantation. 【Methods】Abdominal ectopic heart transplantations were performed using BALB/C mice as donors and C57BL/6 mice as recipients. Mice in the control group did not receive any treatment other than transplantation. For mice in ECP treated group, donor splenocytes suspension was treated by ECP and then injected into recipients via penis vein 7 days before transplantation. The graft survival was assessed by abdominal palpation after transplantation. The spleen were harvested on day 7 for further flow cytometry test; and the H&E staining and immunohistochemistry staining of T lymphocyte of graft tissue was evaluated. 【Results】The graft survival time in the ECP treated group was significantly prolonged compared to the control group（20±4.67 d vs 7.5±0.70 d，P＜0.001）. The frequency of effective T cells in the spleens of recipients in ECP treated group was significantly decreased compared to in the spleens of recipients in the control group. The frequency of CD4+CD25+ Foxp3+ regulatory T cells in the spleen of ECP treated mice was higher than that of control group mice. 【Conclusion】Infusion of ECP treated donor lymphocytes prolongs graft survival after murine heart transplantation, possibly via suppressing effector T cell reaction and induction of regulatory T cell generation.
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Received: 05 July 2016
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2016, Vol. 33 
