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| Experimental Study of The Expression Levels of MMPs in Bundles of Anterior Cruciate Ligament |
| Department of Joint Surgery, Orthopaedic Center, Hunan Provincial People's Hospital, Changsha 410005,China |
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Abstract 【Objective】To detect the expression level of MMPs in the anteromedial and posterolateral bundles of anterior cruciate ligament and to explore the potential place for clinical tendon reconstruction of anterior cruciate ligament. 【Methods】The knees and anterior cruciate ligaments (ACL) were cut out from 10 fresh cadavers that were donated voluntarily. The total RNA was extracted and reverse transcribed into cDNA, then realtime quantitative PCR was used to detect the MMP genes associated with tendon recovery in the anteromedial and posterolateral bundles of the ACL . Meanwhile the samples of anteromedial and posterolateral bundles of the anterior cruciate ligament were fixed and paraffinembedded. Immunohistochemistry experiments were conducted using the MMP2 and MMP3 polyclonal antibodies. Finally the anteromedial and posterolateral bundles of the anterior cruciate ligament were collected, and the total protein was extracted after homogenizing the bundles. MMP2 and MMP3 expression were analyzed between the different bundles by Western blotting. 【Results】There were significant differences for the expression of MMP2 and MMP3 in the anteromedial and posterolateral bundles of the ACL, but there were no significant differences for the expression of the genes MMP1, MMP10, MMP13, and MMP23 in each bundle of ACL. The detection of MMPs in the anterior cruciate ligament using immunohistochemistry and westernblot analysis showed that the expression level of MMP2 is higher in the anteromedial bundle than posterolateral, and that MMP3 expression level is lower in the anteromedial bundle than posterolateral. 【Conclusion】The anteromedial bundle of the anterior cruciate ligament has a better ability to reconstruct itself, and the front of its intersection on tibia plateau is likely to be the the sites of potential clinical tendon reconstruction.
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Received: 15 March 2016
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Corresponding Authors:
通讯作者，王愉思Email:13487490749@163.com
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2016, Vol. 33 
