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Abstract 【Objective】To observe the effects of allicin on RhoA activity and axonal growth in neuronlike PC12 cells and to explore the relationship between allicinpromoting PC12 cells axon growth and RhoA/ROCK signaling activity. 【Methods】 The effects of Allicin on the growth of PC12 cells were detected by MTT assay. The low, medium, and high concentrations of Allicin, which had no effect on the growth of PC12 cells, were selected. PC12 cells were cultured in medium with LPA to induce the activation of RhoA/ROCK signaling pathway and intervened with low, medium, and high concentrations of Allicin. Western blotting was used to detect the expression of ROCK1, ROCK2, RhoA, NF, synapsin and synaptophysin. 【Results】The three Allicin concentrations (10 ng/ml, 100 ng/ml and 1 μg/ml) had no effect on the growth of PC12 cells. The expression of RhoA, ROCK1 and ROCK2 were significantly increased and the expression of NF, synapsin and synaptophysin were dramatically reduced in PC12 cells treatment with LPA. Allicin could effectively reduced RhoA, ROCK1 and ROCK2 expression and recover the expression of NF, synapsin and synaptophysin. 【Conclusion】Allicin intervention can effectively alleviate RhoA activation and inhibitory effects for axon growth induced by LPA. Allicin can promote the growth of axons through inhibiting the activation of RhoA.
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Received: 08 November 2015
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2016, Vol. 33 
