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| Effect of Danshen Powder Injection on the Expression of Autophagyrelated Protein in the Peripheral Blood of Patients with Earlystage of Diabetic Nephropathy |
| Department of Nephrology,Xinghua People's Hospital，Xinghua of Jiangsu province,225700,China |
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Abstract 【Objective】To study the effect of Danshen powder injection on the expression of autophagyrelated protein in the peripheral blood of patients with earlystage of diabetic ephropathy. 【Methods】 Sixtyone patients with early diabetic nephropathy were divided into control group and treatment group according to the patients' willing. Both of the two groups were treated with glimepiride tablets 2 mg once a day, while the treatment group were given with Danshen powder injection 1200mg plus xylitol 250 mL intravenous drip once a day additionally. The treatment course covered 14 d, and two consecutive courses of treatment were conducted. The patients of two groups were detected before and after 24 h ,the urinary albumin excretion amount (UAER), 24 h urine total protein (Upro), serum creatinine (SCr) and blood urea nitrogen (BUN) and glomerular filtration rate (eGFR) and peripheral blood of autophagy associated protein p62 and LC3 expression levels were compared.【Results】The 24h UAER, 24 h Upro, BUN, SCr and eGFR in the control group had no statistical significance compared with those before treatment (P>0.05), but after treatment, the UAER,24hUpro、BUN、SCr and eGFR of the observation group was significantly decreased compared with those before treatment (P<0.05); The expression level of LC3, mRNA and p62 antibody positive rate of the control group had no significant difference before and after treatment (P>0.05); the expression level of LC3 mRNA of the treatment group was significantly increased compared to that before treatment (P<0.05), the p62 antibody positive rate was significantly decreased compared with that before treatment (P<0.05).【Conclusion】Danshen powder intravenous infusion has protective effect on kidney in early stage of DN, and its mechanism may be related to the intervention of autophagy.
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Received: 22 December 2015
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2016, Vol. 33 
