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Abstract 【Objective】To investigate the cerebral protection effect of mild hypothermia and timing choice for rats after cardiopulmonary resuscitation in anoxia. 【Methods】Thirtysix cases of SD mice were randomly divided into shamoperated group (A Group,n=6), normothermia group (B Group ,n=6) and mild hypothcrmia treatment group (C Group,n=24). C group was randomly divided into four subgroups of C1, C2, C3 and C4 groups, with 6 in each group. Rats in A Group just received anesthesia, tracheotomy, vascular access, without cardiopulmonary resuscitation in anoxia; rats in B Group were not treated with mild hypothcrmia; rats in C group were treated with cardiopulmonary resuscitation in anoxia at 2, 4, 6 and 8 min. immediately after heart arrest, respectively. The resuscitation successful rate of each group were recorded. Immunohistochemical method were used to determine the expression of Nrf2 and HO1 protein at cortex and hippocampus. Water content in brain tissue was calculated with Elliott method. 【Results】The expression of Nrf2 and HO1 protein at cortex and hippocampus of C Group was significantly higher than that of the other two groups (P<0.05). No significant difference was found among C1,C2,C3 and C4 Group (P>0.05). Water content in brain tissue of B Group and C Group was significantly higher than that of A Group (P<0.05), but there was no significant difference between B group and C group (P>0.05), and there was no significant difference in the four subgroups of C1, C2, C3 and C4 (P>0.05).【Conclusion】 The cerebral protection effect of mild hypothcrmia for rats after cardiopulmonary resuscitation in anoxia may be realized through Nrf2ARE signaling pathway, by upregulating the expression level of Nrf2 and HO1. Mild hypothermia treatment should be carried out in the early stage of cardiac arrest after cardiac resuscitation in order to relieve the brain injury and improve the success rate of cardiopulmonary resuscitation.
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Received: 26 October 2015
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2016, Vol. 33 
