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| Impact of 1, 25 dihydroxycholecalciferol on Kidney Injury of Type 2 Diabetic Rats |
| Department of Geriatrics,First College of Clinical Medical Science,China Three Gorges University,Central People's Hospital of Yichang,Yichang Hubei 443003,China |
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Abstract 【Objective】To study the impact of 1, 25 dihydroxycholecalciferol on kidney injury of type 2 diabetic rats. 【Methods】30 male Wistar rats were randomly divided into normal diet group (N group, 10 rats) and high fat and high glucose diet group (model group, 20 rats), Type 2 diabetes model group were successfully set and randomly divided into two groups, with 10 rats in each group, diabetes mellitus group (M group) was treated by intraperitoneal injection of 2.5 g/(kg•d) of peanut oil, and D group by intraperitoneal injection 2.5 μg/(kg•d) 1, 25 dihydroxycholecalciferol dissolved in peanut oil. After 8 weeks, blood samples were collected to detect blood glucose, creatinine and urea nitrogen;At the same time, the rats were sacrificed and the kidney tissues were kept.Tumor necrosis factor alpha (TNFα), transforming growth factor beta 1 (TGFβ1) were detected by Blot Western and compared with that in renal tissue.【Results】Blood glucose, urea nitrogen and creatinine in M group and D group were significantly higher than those in N group (P<0.05), but the blood glucose, urea nitrogen and creatinine in D group were significantly lower than that in M group (P<0.05); The levels of TNFα、TGFβ1 in M group and D group were significantly higher than those in N group, while the D group was significantly lower than that in M group (P<0.05).【Conclusion】1, 25 dihydroxycholecalciferol can inhibit expression of TNFα、TGFβ1 of diabetic rats kidney and take protective effect on renal injury in rats with diabetes.
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Received: 09 February 2015
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2015, Vol. 32 
