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Abstract [Objective]To explore the expression of miRNA‐29b (miR‐29b) ,examine its effects on cell bi‐ological properties in prostate cancer (Pca) and discuss its potential clinical values .[Methods]The expressions of miR‐29b in prostate cancer tissues and matched adjacent non‐tumor tissues were detected by real‐time quan‐titative polymerase chain reaction (QRT‐PCR) .The cellular growth and chemotherapeutic effect of cisplatin were measured by methyl thiazol tetrazolium (MTT) assay .Flow cytometry was used to determine the cell cy‐cle changes .And AV/PI double labeling method was utilized to detect the apoptosis of LNCap .Finally the im‐pact of miR‐29b on the invasiveness of LNCap cell was determined by Transwell invasion assay .[Results]The expression of miR‐29b was down‐regulated in Pca tissues versus matched adjacent non‐tumor tissues .Com‐pared with negative controls ,group of transfected miR‐29b showed lower tumor cell survival rate .Moreover , miR‐29b could increase the inhibition of cisplatin on LNCap with much lower cell survival rate .LNCap with transfected miR‐29b revealed a higher percentage of cells during sub‐G0/G1 phase and significant decreases of apoptosis cell rate and invasive cell rate ( P <0 .05) .[Conclusion]The expression of miR‐29b is down‐regula‐ted in prostate cancer .A higher expression of miR‐29b inhibits cell proliferation and invasion and induces cell apoptosis in prostate cancer .And the chemosensitivity of cisplatin increases in Pca cell .
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2015, Vol. 32 
