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Abstract To explore the effect of triacontanol(TRIA) on myocardial ischemia-reperfusion in-jury(IR) in isolated rat cardiac IR model .[Methods]Langendorff perfusion device was used to establish rat i-solated heart IR model .All rats were randomly divided into normal group ,different dose TRIA+IR group and TRIA(2 .5 g/ml) group .The normal group was given whole heart continuous perfusion for 90min .The IR group was given the balance for 30min and then reperfusion for 30min after 30min-ischemia .The TRIA-IR group was given 25 ,15 ,5 and 2 .52 .5 g/ml TRIA at 5min before ischemia and then the same treatment as IR group .The TRIA group was given the medication for 5min after 30min-balance and then continuous perfusion for 30min .Heart rate(HR) ,coronary flow (CF) ,left ventricular pressure (LVP) and maximum velocity of LVP variation(dp/dtmax ) were observed .The level of creatine kinase(CK) in coronary effluent was measured .[Results]Compared with normal group ,IR could induce the obvious heart dysfunction as shown by a decrease in HR ,CF ,LVP and dp/dtmax and an increase in CK level .Heart perfusion with different doses of TRIA (25 , 15 ,5 and 2 .5 g/mL) for 5min aggravated IR-induced cardiac dysfunction and even heart arrest as shown by a further decrease in HR ,CF ,LVP and dp/dtmax and an increase in CK level .TRIA(2 .5 g/mL) alone also in-hibited rat cardiac function which was not recovered for continuous 30min .[Conclusion]TRIA can inhibit cardi-ac function and aggravate myocardial IR injury in Langendorff rat .
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2014, Vol. 31 
