|
Abstract Objective To explore the effect and mechanism of pioglitazone for the treatment of nonalcoholic fatty liver disease(NAFLD) in rats .[Methods] Thirty six male SD rats were randomly divided into normal control group (group NG) ,pioglitazone treatment group(group PIOG) and high-fat diet group(group FG) .All rats were fed for 12 weeks .Group NG was fed with standard diet ,and the rest two groups were fed with high-fat diet .Group PIOG was giv-en pioglitazone by lavage at 8~12 weeks ,while other 2 groups were given distilled water by lavage in the same period . The levels of fasting serum alanine aminotransferase(ALT) ,aspartate aminotransferase(AST) ,triglycerides(TG) ,total cholesterol(TC) ,fasting glucose(FPG) ,fasting blood insulin(FINS) ,fasting insulin resistance index (FIRI) ,tumor nec-rosis factor-α(TNF-α) and nitric oxide(NO) were compared among 3 groups .HE staining method was used to analyze the pathologic change of hepatic tissue section .The morphological change of Kupffer cells (KCs) was observed .[Results]The levels of FIRI ,TG and TC in group FG were higher than those in group NG ,and there was significant difference ( P<0 .05) .Hepatic tissues showed big bubble fatty degeneration ,inflammatory cell infiltration and dot-like necrosis .KCs in liver tissue had morphological change .The levels of TNF and NO produced by KCs were positively correlated with pathological changes of liver tissue( P<0 .05) .The levels of FIRI ,TG and TC in group PIOG were lower than those in group FG ,and there was significant difference ( P<0 .05) .The degree of liver steatosis was improved ,but the inflam-matory cell infiltration and hepatocyte ballooning degeneration were still visible .The shape and function of liver KCs were still abnormal .[Conclusion]Pioglitazone can partly delay the development of NAFLD induced by high-fat diet .The mech-anism may be associated with the improvement of insulin resistance and the decreasing of blood lipids ,but not related with the regulation of KCs .
|
|
|
|
|
|
2014, Vol. 31 
