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Abstract Objective To explore biological characteristics of wide tropism pseudomonas aeruginosa (PA ) in order to provide the ideas for PA infection control .[Methods]PA phages were separated from sewer of five hospi-tals .After PA phages were purified by double-agar negative colony formation method ,wide tropism phages were screened .The characteristics were observed and one-step growth line was drawn .Nucleic acid was extracted for enzyme digestion analysis .[Results] Totally 8 strains of PA phages were isolated .Three strains of wide tropism phages were screened ,in which PAp-3 showed wider phage spectrum .PAp-3 was transparent ,round and clear boundary plaque .PAp-3 genome was 23kb .When MOI was 0 .001 ,the titer of progeny PA phage produced by the infected host was highest(6 .4 × 1010 PFU/mL) .The incubation period of the infected host bacteria was 30min ,and the outbreak time was 55min ,and the burst size was 100 .The engulfed coverage rate of cross combination of 3 wide tropism phages on 20 strains of the isolated PA phages was 95 .00% (19/20) .[Conclusion] Wide tropism phage has wider spectrum ,shorter incubation period large burst size ,higher engulfed coverage rate of cross combi-nation ,and can provide the basis for the phage used as antibacterial biological agents .
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2014, Vol. 31 
