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Abstract [Obj ective]To explore the differential expression of proteomics of eosinophilic bronchitis mouse model and asthma mouse model.[Methods]Four rats in each group including eosinophilic bronchitis model(EB model)(experimental group),asthma model(asthma group)and control group.Total protein was extracted from lung tissue.Matrix-assisted laser desorption/ionization time of flight mass spectrometry was used for sequence a-nalysis of proteins.[Results]Two dimensional electrophoresis image showed that there was difference in the ex-pression of protein among 3 groups(P<0.05).Twenty proteins such as glutathione-S-transferase M1(GSTM1) and heat shock protein B1(HSPB1)were identified through the analysis of peptide mass fingerprint spectrum of mass spectrometry contrasted with standard molecular weight and isoelectric point.GSTM1 was down-regulated in asthma group,while HSPB1 was up-regulated in asthma group.[Conclusion]GSTM1 and HSPB1 may involve in the occurrence of airway hyperresponsiveness.
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2014, Vol. 31 
