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| Effect of Ulinastatin on IL-1 7 in Brain Tissues of Rats with Cerebral Ischemia-reperfusion Inj u-ry |
| REN Xiang%YANG Jie%LI li |
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Abstract [Objective]To explore the protective effect and mechanism of ulinastatin(UTI)against cerebral ischemia-reperfusion injury.[Methods]A total of 72 male SD rats weighed 200~250g and aged 10~12 weeks were randomly divided into 3 groups including sham operation group,model group and UTI-treated group. The model of cerebral ischemia-reperfusion inj ury in model group and UTI-treated group were made by occlu-ding middle cerebral artery with thread,and ischemic time was 60min.Sham operation group only underwent neck surgery without occluding the vessel. UTI-treated group received intraperitoneal inj ection of UTI (10000U/kg)daily.The model group and sham group received the injection of isovolume normal saline daily. Petullo score was used to evaluate nervous function of 3 groups at 3d,7d and 14d after ischemia-reperfusion. Immunohistochemistry and Western blot were used to detect the expression of interleukin-17(IL-17)in rats of 3 groups at 3d,7d and 14d after ischemia-reperfusion.The effect of UTI on nervous function and IL-17 in brain tissues of rats with cerebral ischemia-reperfusion was observed.[Results]There were significant differ-ences in Petullo scores at d3 ,d7 and d14 between UTI-treated group and model group[(2.87±0.25)vs.(5.00 ±0.91),(2.20±1.52)vs.(4.87±0.62),(2.00±0.50)vs.(4.50±0.81)](P<0.05).Compared with the model group,the expression of IL-1 7 in UTI-treated group was obviously decreased,and there were signif-icant differences in the expression of IL-17 at d3 ,d7 and d 14 between two groups(P<0.05).[Conclusion]UTI may alleviate neurologic impairment after cerebral ischemia-reperfusion by decreasing the expression of IL-17.
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2014, Vol. 31 
