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Abstract [Objective]To explore the influence of simulated microgravity on differentiation of CD34+cells.[Methods]CD34+ cells were cultured by fluid medium in the simulated microgravity condition created by the rotary cell-culture systems(RCCS)and normal gravity condition.The same fluid medium in 1-g condition was taken as the culture group(1-g fluid group),while the standard methylcellulose semisolid medium was taken as control group(1-g methykcekkulose group).Flow cytometry was used to detect the percentage of spe-cific antigen of cells in each group.The effect of simulated microgravity on differentiation of CD34+ cells was analyzed.Meanwhile,propidium iodide(PI)cell apoptosis staining was performed.The effect of simulated mi-crogravity on apoptosis of CD34+ cells was analyzed.[Results]Flow cytometry showed that CD34+ cells in each group were less than 0.2%.The expression of erythroid cell(GlyA+)in RCCS group was obviously low-er than that in 1-g liquid control group(22.21%±3.02% vs.60.05%±3.08%,P<0.05),and the expres-sion levels of CD34+ in RCCS group and 1-g liquid control group were 52.12%±1.92% and 18.87%±1.41%respectively,and there was significant difference between two groups(P <0.05).The expression level of GlyA+(54.39%±2.86%)and CD34+(21.09%±3.19%)in 1-g liquid control group were similar to those in 1-g methylcellulose group,and there was no significant difference between two groups(P>0.05).The pro-portion of apoptotic cells in each group was less than 1%.[Conclusion]The simulated microgravity can inhibit the differentiation of CD34+ to all blood cells,especially inhibit the differentiation of CD34+ cells to erythroid cells.
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2014, Vol. 31 
