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| Construction and Identification of Adenovirus Vector of Promoter/Enhancer Sequences of ap2 and AQP7 cDNA |
| BAI Xiao-su%LU Ze-yuan%SHAO Hao |
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Abstract [Objective]To construct fusion protein expression vector pEGFP-ap2-AQP7 of promoter/enhancer sequences of ap2 and AQP7 cDNA.[Methods]The products of splicing promoter/enhancer of ap2 and AQP7 cDNA were cloned into pIRES2-EGFP.Adenovirus vector recombinant fusion protein was obtained.The infected 3T3 fat cell was used to determine the infextation effect.[Results]① The pEGFP-ap2-AQP7 fusion protein was expressed in the constructed cell strains stably.The enhanced green fluorescent protein was used as a marker.The positio-ning of AQP7 in 3T3 fat cells were observed.[Conclusion]The stable construction of 3T3 fat cell strains express-ing fusion protein pEGFP-ap2-AQP7 can provide the basis for intracellular localization and function research of AQP7 .
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2014, Vol. 31 
