Abstract：【Objective】 To investigate the protective effect of emodin on alveolar epithelial cell injury induced by Streptococcus pneumoniae and its mechanism. 【Methods】A549 alveolar epithelial cells were randomly divided into control group, model group and 20 μg/mL group, 40 μg/mL group, 80 μg/mL group, 160 μg/mL group, the cells in the control group were cultured routinely, and the cells in the other groups were infected by Streptococcus pneumoniae; 20 μg/mL group, 40 μg/mL group, 80 μg/mL group, 160 μg/mL group cells were added with different concentrations of emodin (20 μg/mL,40 μg/mL,80 μg/mL,160 μg/mL). The proliferation of A549 cells was detected by MTT; The apoptosis rate was detected by flow cytometry; Level of interleukin-6 (IL-6) and tumor necrosis factor-α(TNF-α)was detected by enzyme linked immunosorbent assay (ELISA) ; In A549 cells, B cell lymphoma / leukemia-2 (Bcl-2), Bcl-2 associated X protein (Bax), cleaved caspase containing cysteine-3(Cleaved Caspase-3),β-catenin and the protein levels of Cyclin D1 were detected by Western blot. 【Results】 Compared with the control group,the protein levels of the proliferative activity, Bcl-2, β-catenin and cyclin D1 decreased (P<0.05); The levels of apoptosis rate, IL-6 and TNF-α, Bax and cleared Caspase-3 protein increased (P<0.05); Different concentrations of emodin could promote the proliferation of A549 cells infected by Streptococcus pneumoniae (P<0.05), inhibit its apoptosis (P<0.05), and reduce IL-6 and TNF-α level (P<0.05), increase Bcl-2 protein level (P<0.05), decrease Bax and Cleaved Caspase-3 protein level (P<0.05) and increase protein levels of β-catenin and cyclin D1 (P<0.05). 【Conclusion】 Emodin may regulate Wnt/β-catenin signaling pathway, promote the proliferation of alveolar epithelial cells infected by Streptococcus pneumoniae and inhibit their apoptosis.
孙建荣, 赵静, 刘文林. 大黄素对肺炎链球菌感染的肺泡上皮细胞损伤的作用及其机制研究[J]. 医学临床研究, 2022, 39(7): 1006-1009.
SUN Jian-rong, ZHAO Jing, LIU Wen-lin. Protective Effect of Emodin on Alveolar Epithelial Cell Injury Induced by Streptococcus Pneumoniae and its Mechanism. JOURNAL OF CLINICAL RESEARCH, 2022, 39(7): 1006-1009.
[1] ANDRADE D C,BORGES I C,VILAS-BOAS A L,et al. Infection by Streptococcus pneumoniae in children with or without radiologically confirmed pneumonia[J].J Pediatr,2018,94(1):23-30.
[2] LIU Z,MA N,ZHONG Y,et al. Antiviral effect of emodin from Rheum palmatum against coxsakievirus B 5 and human respiratory syncytial virus in vitro[J].J Huazhong Univ Sci Technolog Med Sci,2015,35(6): 916-922.
[3] TIAN S L,YANG Y,LIU X L,et al. Emodin attenuates bleomycin-induced pulmonary fibrosis via anti-inflammatory and anti-oxidative activities in rats[J].Med Sci Monit,2018,24:1-10.
[4] SUN Y,WANG X,ZHOU Q,et al. Inhibitory effect of emodin on migration,invasion and metastasis of human breast cancer MDA-MB-231 cells in vitro and in vivo[J].Oncol Rep,2015,33(1):338-346.
[5] SHI S,LIU X,LI H. Downregulation of caspase-3 alleviates Mycoplasma pneumonia-induced apoptosis in alveolar epithelial cells[J].Mol Med Rep,2017,16(6):9601-9606.
[6] PEÑA-BLANCO A,GARCÍA-SÁEZ A J. Bax,Bak and beyond-mitochondrial performance in apoptosis[J].FEBS J,2018,285(3): 416-431.
[7] RENAULT T T,DEJEAN L M,MANON S. A brewing understanding of the regulation of Bax function by Bcl-xL and Bcl-2[J].Mech Ageing Dev,2016,161 (Pt B):201-210.
[8] FIANDALO M V,KYPRIANOU N. Caspase control: protagonists of cancer cell apoptosis[J].Exp Oncol,2012,34(3): 165-175.
[9] BRENTNALL M,RODRIGUEZ-MENOCAL L,DE GUEVARA R L,et al. Caspase-9,caspase-3 and caspase-7 have distinct roles during intrinsic apoptosis[J].BMC Cell Biol,2013,14(1): 32.
[10] 谢璟,王荣丽. 大黄素对肺炎链球菌肺炎小鼠肺组织炎症反应及p38 MAPK表达的影响[J].山东医药,2018,58(3):44-47.
[11] GUO X,CHEN Y,HONG T,et al. Induced pluripotent stem cell-derived conditional medium promotes Leydig cell anti-apoptosis and proliferation via autophagy and Wnt/β-catenin pathway[J].J Cell Mol Med,2018,22(7): 3614-3626.
[12] QU Z,SU F,QI X,et al. Wnt/β-catenin signalling pathway mediated aberrant hippocampal neurogenesis in kainic acid-induced epilepsy[J].Cell Biochem Funct,2017,35(7): 472-476.
[13] GU J,CUI C F,YANG L,et al. Emodin inhibits colon cancer cell invasion and migration by suppressing epithelialmesenchymal transition via the Wnt/β-catenin pathway[J].Oncol Res,2018,27(2):193-202.
2022, Vol. 39 
