Expression and Significance of Phosphatidylinositol Kinase-3 and Protein Kinase Akt in Mkeletal Muscle of Postmenopausal Hip Fracture Patients
Mirarimu Multiza, LIN Hai-Shan, Eniwalj Da Maura, et al
Department of Orthopedic, Joint and Geriatric Osteopathy,The People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi City, Xinjiang Uygur Autonomous Region, 830001
Abstract：【Objective】 To investigate the expression levels and clinical significance of phosphatidylinositol kinase-3 (PI3K) and protein kinase (Akt) in skeletal muscle of postmenopausal hip fracture patients. 【Methods】 Prospectively selected a total of 80 post-menopausal patients who underwent surgery for hip fracture in our department from January 2017 to June 2019. Patients were divided into 4 different groups according to the dual-energy X-ray bone density (DXA) levels. They were normal bone mass group (-1≤T≤1, group A), bone mass reduction group (-2.5 <T <-1, group B), osteoporosis Group (T ≤-2.5, group C) and severe osteoporosis group(T ≤-2.5 with one or more brittle fractures, group D).A small amount of muscle tissue was taken from the lateral femoral muscle during the operation for RNA extract, quantitative Real-time PCR (RT-PCR) was detected for mRNA expression level of phosphatidyl inositol kinase- 3(PI3K)and protein kinase (Akt), while the protein expression levels of PI3K and Akt were measured by immunohistochemical technique. The correlation of PI3K and Akt with the bone density was analyzed as well. 【Results】 Both mRNA and protein expression levels of Akt and PI3K in the group D were higher than those in other groups (P<0.05).The mRNA and protein expression levels of Akt and PI3K in the group C was higher than that that in the group B (P<0.05). However, the mRNA and protein expression levels of Akt and PI3K were not statistically significant difference between the group A and B (P>0.05). The expression levels of AKT and PI3K were positively correlated with the bone mineral density of the patients. 【Conclusion】 The PI3K/Akt signaling pathway is activated in skeletal muscle of postmenopausal osteoporosis patients, and the mRNA and protein expression levels of PI3K and Akt are increased and also positively correlated with the degree of osteoporosis. It suggests that PI3K/Akt plays an important role in the regulation of bone-muscle formation and reconstruction.
米尔阿里木·木尔提扎, 蔺海山, 艾尼瓦尔江·达毛拉, 韩衍龙, 赵巍, 袁宏, 王利. 磷脂酰肌醇激酶-3、蛋白激酶在绝经后髋部骨折患者骨骼肌组织中的表达及其临床意义[J]. 医学临床研究, 2020, 37(5): 664-667.
Mirarimu Multiza, LIN Hai-Shan, Eniwalj Da Maura, et al. Expression and Significance of Phosphatidylinositol Kinase-3 and Protein Kinase Akt in Mkeletal Muscle of Postmenopausal Hip Fracture Patients. JOURNAL OF CLINICAL RESEARCH, 2020, 37(5): 664-667.
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2020, Vol. 37 
