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| 脂肪间充质干细胞治疗大鼠慢性阻塞性肺疾病的实验研究 |
| 张晋, 孙良璋, 李少民, 张丹杰 |
| 西安交通大学第二附属医院胸外科,陕西 西安 710004 |
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摘要 【目的】探讨脂肪间充质干细胞(ADMSCs)治疗大鼠慢性阻塞性肺疾病(COPD)的机制。【方法】分离、培养大鼠ADMSCs,烟雾暴露法建立COPD大鼠模型,然后分组分别经大鼠尾静脉给予不同浓度ADMSCs治疗。观察各浓度组肺组织病理学变化,检测各组肺泡灌洗液细胞计数、白细胞介素--6(IL-6)、IL-8、肿瘤坏死因子-α(TNF-α)、抗氧化指标超氧化物歧化酶(SOD)及丙二醛(MDA)水平并比较。【结果】ADMSCs静脉注射可减轻大鼠肺组织病理损伤,减少肺泡灌洗液中细胞数目,使血清中的SOD水平增加,MDA 、TNF-α、IL-8水平降低。【结论】ADMSCs治疗大鼠COPD疗效显著,可减轻其肺泡组织损伤和炎性细胞浸润。
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| 关键词 ：
肺疾病慢性阻塞性/治疗,
间充质基质细胞,
大鼠Wistar
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收稿日期: 2019-05-05
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通讯作者:
E-mail:zhangjinxjtu@163.com
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2019, Vol. 36 
