Abstract：【Objective】To investigate the effects of propofol or sevoflurane combined with remifentanil on myocardial ischemia-reperfusion injury (IRI) in rats.【Methods】 Sixty clean rats were randomly divided into 5 groups: ischemia grougroup, sevoflurane group, propofol group, ischemia reperfusion group (I/R group) and sham operation group (SO group) with 12 in each group. The IRI of rats in vitro was established according to the classical method.In each group, myocardial infarction area index, apoptosis index Toll-like receptor 2 (TLR-2) Toll-like receptor 4 (TLR-4), tumor necrosis factor-α (TNF-α), nuclear transcription factor- κ B (NF-κB), ribosomal protein s6 kinase (p-p70S6K) and cardiac function indexes left ventricular end-diastolic pressure (LVEDP), left ventricular maximum rate of decline (-dp/dt), left ventricular maximum rate of rise] (+dp/dt), heart rate (HR), left ventricular progressive pressure (LVDP) were compared.【Results】 In the propofol group and the sevoflurane group, there was no significant difference in LVEDP, LVDP and HR compared with those in the SO group (P>0.05), the +dp/dt, -dp/dt, LVDP in the propofol group and the sevoflurane group was significantly higher than those in the ischemic group. but lower than those of the I/R group; LVEDP was significantly lower than that in the ischemia group and the I/R group (P<0.05), and the HR was significantly higher than that in the ischemia group and the I/R group (P<0.05),and there was significant difference between propofol group and sevoflurane group (P<0.05). There was no significant difference in the TLR-2, TLR-4, TNF-α, NF-κB protain, p-p70S6K protein expression between the propofol groeup, the sevoflurane group and the SO group (mean P>0.05). The indexes of the propofol group and the sevoflurane group were significantly higher than that of the ischemia group and lower than that of the I/R group (P<0.05),there was significant difference between the propofol group and the sevoflurane group (P<0.05). The myocardial infarction area index and apoptosis index in the propofol group and the sevoflurane group were significantly higher than those in the SO group and lower than that in the ischemia group and the I/R group (P<0.05).【Conclusion】For myocardial IRI rats, propofol combined with remifentanil anesthesia could protect cardiac function, decrease myocardial infarction area index, ameliorate the imbalance of mediators, promote the p-p70S6K,NF-κB, TLR-2, TNF-α, TLR-4 expression and decrease the apoptosis index of rat heart in vitro.
张亮, 孙虎, 徐志新. 异丙酚或七氟烷复合瑞芬太尼对大鼠心肌缺血再灌注损伤的影响[J]. 医学临床研究, 2018, 35(6): 1069-1071.
ZHANG Liang. Study on the Effects of Remifentanil Combined With Propofol or Sevoflurane for Myocardial Ischemia-reperfusion in Rats. JOURNAL OF CLINICAL RESEARCH, 2018, 35(6): 1069-1071.
2018, Vol. 35 
